Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Affinity purified
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag)
See all SARS Nucleocapsid Protein proteins and peptides -
Purity
> 95 % Affinity purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Sequence
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTA SWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRN PANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPG SSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKS AAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKH WPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQV ILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADL DDFSKQLQQSMSSADSTQARNPANNAAIVLQLPQGTTLPKGFYAEGSRGG SQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQ LESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGP EQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGT WLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADET QALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA -
Molecular weight information
48 kDa by SDS-PAGE -
Amino acids
1 to 419 -
Tags
His tag C-Terminus -
Additional sequence information
Isolate Wuhan-Hu-1
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Description
Recombinant Human coronavirus SARS Nucleocapsid Protein (His tag)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab272107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Recombinant Human coronavirus SARS CoV-2 Nucleocapsid Protein (His tag) tends to aggregate in solution.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.00
Constituent: 99% DPBS
General Info
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Alternative names
- N
- NC
- Nucleocapsid protein
see all -
Relevance
Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). SARS is caused by a human coronavirus, which are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. The nucleocapsid protein of SARS shares little homology with nucleocapsid proteins of other members of the coronavirus family. Nucleocapsid proteins of other coronavirus have been reported to be involved in forming the viral core and also in the packaging and transcription of the viral RNA. -
Cellular localization
Inside the virion, complexed with the viral RNA. May be associated with cellular membranes where it participates in viral RNA synthesis and virus budding.
Images
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SDS-PAGE - Recombinant Human coronavirus SARS CoV-2 Nucleocapsid Protein (His tag) (ab272107)
SDS-PAGE analysis of ab272107.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab272107 has been referenced in 2 publications.
- Urbán P et al. The SARS-CoV-2 Nucleoprotein Induces Innate Memory in Human Monocytes. Front Immunol 13:963627 (2022). PubMed: 35928816
- Hatakeyama D et al. In-vitro acetylation of SARS-CoV and SARS-CoV-2 nucleocapsid proteins by human PCAF and GCN5. Biochem Biophys Res Commun 557:273-279 (2021). PubMed: 33894414