Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, ELISA, WB
Description
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Product name
Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)
See all SARS-CoV-2 nucleocapsid protein proteins and peptides -
Purity
>= 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Sequence
MSDNGPQNQR NAPRITFGGP SDSTGSNQNG ERSGARSKQR RPQGLPNNTA SWFTALTQHG KEDLKFPRGQ GVPINTNSSP DDQIGYYRRA TRRIRGGDGK MKDLSPRWYF YYLGTGPEAG LPYGANKDGI IWVATEGALN TPKDHIGTRN PANNAAIVLQ LPQGTTLPKG FYAEGSRGGS QASSRSSSRS RNSSRNSTPG SSRGTSPARM AGNGGDAALA LLLLDRLNQL ESKMSGKGQQ QQGQTVTKKS AAEASKKPRQ KRTATKAYNV TQAFGRRGPE QTQGNFGDQE LIRQGTDYKH WPQIAQFAPS ASAFFGMSRI GMEVTPSGTW LTYTGAIKLD DKDPNFKDQV ILLNKHIDAY KTFPPTEPKK DKKKKADETQ ALPQRQKKQQ TVTLLPAADL DDFSKQLQQS MSSADSTQA -
Predicted molecular weight
48 kDa including tags -
Amino acids
1 to 419 -
Tags
His tag C-Terminus -
Additional sequence information
N-terminal MSKIKGS, C-terminal EFGGGS 8xHis
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab273530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
Western blot
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Form
Lyophilized -
Additional notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at -80°C.
Information available upon request.
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ReconstitutionReconstitute in Phosphate Buffered saline, aliquot and store at -80 C for 12 months or +4 C for 1 week. Avoid repeated freeze thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- 2019-nCoV nucleocapsid protein
- N
- NC
see all -
Relevance
Nucleocapsid protein is a most abundant protein of coronavirus on the helical nucleocapsid of coronaviruses. N protein of SARS CoV-2 ab273530 is a structural protein required for RNA synthesis, and has RNA chaperone activity that may be involved in template switch. N protein enters the host cell with the viral RNA to facilitate its replication and process the virus particle assembly and release. N protein is a highly immunogenic phosphoprotein also implicated in modulating cell signalling pathways. Coronavirus nucleocapsid proteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein.
Images
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Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)
Samples
Lanes 1 & 3: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab273530) (0.2 µg)
Lanes 2 & 4: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107) (0.2 µg)
Green: Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein (ab271180)
Red: Mouse monoclonal [HIS.H8] to 6X His tag® (ab18184)
Lanes 1 & 2: Green - ab271180 observed at 50 kDa.
Lanes 3 & 4: Red - loading control Mouse anti-6x HisTag observed at 50 kDa.
ab271180 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3% milk before incubation with ab271180 and Mouse anti-6x HisTag overnight at 4 °C at a 1/1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.
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Western blot - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)Samples
Lanes 1 & 3: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab273530) (0.1 µg)
Lanes 2 & 4: Recombinant Human coronavirus SARS-CoV-2 Nucleocapsid Protein (His tag) (ab272107) (0.1 µg)
Green: Rabbit monoclonal [EPR24334-118] to SARS-CoV-2 nucleocapsid protein - BSA and Azide free (ab280201)
Red: Mouse monoclonal [HIS.H8] to 6X His tag® (ab18184)
Lanes 1 & 2: Green - ab280201 observed at 50 kDa.
Lanes 3 & 4: Red - loading control Mouse anti-6x HisTag observed at 50 kDa.
ab280201 was shown to react with SARS-CoV-2 nucleocapsid protein in Western blot. Membranes were blocked in 3% milk before incubation with ab271180 and Mouse anti-6x HisTag overnight at 4 °C at a 1/1000 dilution. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.
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SDS-PAGE - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)SDS-PAGE analysis of ab273530.
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ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) and Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 500 ng/ml.
Primary antibody (ab271180) concentration range 0.045-100 ng /ml.
Pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) used at 1/2000 dilution.
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ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (ab273530)Plates were coated with Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag, ab273530) and Recombinant Human coronavirus SARS Nucleocapsid Protein (ab270829) at 500 ng/ml.
Primary antibody (ab271180) concentration range 0.045-100 ng /ml.
Pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) used at 1/2000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab273530 has been referenced in 2 publications.
- Sadique MA et al. Highly Sensitive Electrochemical Immunosensor Platforms for Dual Detection of SARS-CoV-2 Antigen and Antibody based on Gold Nanoparticle Functionalized Graphene Oxide Nanocomposites. ACS Appl Bio Mater 5:2421-2430 (2022). PubMed: 35522141
- Sadique MA et al. Functionalized Titanium Dioxide Nanoparticle-Based Electrochemical Immunosensor for Detection of SARS-CoV-2 Antibody. Diagnostics (Basel) 12:N/A (2022). PubMed: 36359457