Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)
Key features and details
- Expression system: CHO cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Indirect ELISA, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)
See all SARS-CoV-2 Spike Glycoprotein S1 proteins and peptides -
Biological activity
Flow cytometry assay showing ab273068 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were stained with ab273068, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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Purity
> 90 % SDS-PAGE.
Purity is lot specific. Please contact our technical Support team for details. -
Expression system
CHO cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Sequence
VNLTTRTQLP PAYTNSFTRG VYYPDKVFRS SVLHSTQDLF LPFFSNVTWF HAIHVSGTNG TKRFDNPVLP FNDGVYFAST EKSNIIRGWI FGTTLDSKTQ SLLIVNNATN VVIKVCEFQF CNDPFLGVYY HKNNKSWMES EFRVYSSANN CTFEYVSQPF LMDLEGKQGN FKNLREFVFK NIDGYFKIYS KHTPINLVRD LPQGFSALEP LVDLPIGINI TRFQTLLALH RSYLTPGDSS SGWTAGAAAY YVGYLQPRTF LLKYNENGTI TDAVDCALDP LSETKCTLKS FTVEKGIYQT SNFRVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF ELLHAPATVC GPKKSTNLVK NKCVNFNFNG LTGTGVLTES NKKFLPFQQF GRDIADTTDA VRDPQTLEIL DITPCSFGGV SVITPGTNTS NQVAVLYQDV NCTEVPVAIH ADQLTPTWRV YSTGSNVFQT RAGCLIGAEH VNNSYECDIP IGAGICASYQ TQTNSPRRAR -
Predicted molecular weight
76 kDa -
Actual molecular weight
125 kDa -
Amino acids
16 to 685 -
Tags
His tag C-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab273068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Indirect ELISA
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Preservative: 1.02% Imidazole
Constituents: 0.82% Sodium phosphate, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Indirect ELISA - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)
Indirect ELISA showing primary antibody ab273073 (CR3022, human chimeric) binding to the antigens ab272105 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (sheep Fc fusion)) and ab273068 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)). Plates were coated with 100ng/well ab272105 or ab273068 and binding of ab273073 assessed in serial dilution from 200ng/ml primary antibody in duplicate. Binding was detected using ab98624, an anti-human Fc secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
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Indirect ELISA - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)Indirect ELISA showing primary antibody ab273074 (CR3022, rabbit chimeric) binding to the antigen ab273068 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)). Plates were coated with 100ng/well ab272105 or ab273068 and binding of ab273073 assessed in serial dilution from 200ng/ml primary antibody in duplicate. Binding was detected using ab97080, an anti-rabbit H&L secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
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Functional Studies - Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S1) (Active) (ab273068)Flow cytometry assay showing ab273068 can bind to ACE2 overexpressing cells. ACE2 overexpressing cells were stained with ab273068, followed by an anti-spike protein antibody, and a fluorescence-conjugated secondary antibody.
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SDS-PAGE - Recombinant SARS Coronavirus Spike Glycoprotein Mosaic (S1) (Active) (ab273068)SDS-PAGE analysis of ab273068.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab273068 has been referenced in 9 publications.
- Brazaca LC et al. Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2. Anal Bioanal Chem 414:5507-5517 (2022). PubMed: 35169906
- Bistaffa MJ et al. Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein. Talanta 244:123381 (2022). PubMed: 35364338
- Emam MH et al. Inhibition of SARS-CoV-2 spike protein entry using biologically modified polyacrylonitrile nanofibers: in vitro study towards specific antiviral masks. RSC Adv 12:16184-16193 (2022). PubMed: 35733688
- Olajide OA et al. Induction of Exaggerated Cytokine Production in Human Peripheral Blood Mononuclear Cells by a Recombinant SARS-CoV-2 Spike Glycoprotein S1 and Its Inhibition by Dexamethasone. Inflammation N/A:N/A (2021). PubMed: 33860869
- Passariello M et al. Novel human neutralizing mAbs specific for Spike-RBD of SARS-CoV-2. Sci Rep 11:11046 (2021). PubMed: 34040046
- Constant O et al. SARS-CoV-2 Poorly Replicates in Cells of the Human Blood-Brain Barrier Without Associated Deleterious Effects. Front Immunol 12:697329 (2021). PubMed: 34386007
- Kozlovskaya LI et al. Long-term humoral immunogenicity, safety and protective efficacy of inactivated vaccine against COVID-19 (CoviVac) in preclinical studies. Emerg Microbes Infect 10:1790-1806 (2021). PubMed: 34427172
- Olajide OA et al. Garcinia kola and garcinoic acid suppress SARS-CoV-2 spike glycoprotein S1-induced hyper-inflammation in human PBMCs through inhibition of NF-κB activation. Phytother Res 35:6963-6973 (2021). PubMed: 34697842
- Pinto G et al. Identification of SARS-CoV-2 Proteins from Nasopharyngeal Swabs Probed by Multiple Reaction Monitoring Tandem Mass Spectrometry. ACS Omega 6:34945-34953 (2021). PubMed: 34926968