Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (mutated Q677H) (Active) (ab289629)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (mutated Q677H) (Active)
See all SARS-CoV-2 Spike Glycoprotein S1 proteins and peptides -
Biological activity
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 µg/mL (100 µL/well) can bind ab289629, the EC50 of ab289629 is 100-600 ng/mL.
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Purity
> 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human coronavirus -
Predicted molecular weight
77 kDa -
Molecular weight information
ab289629 consists of 681 amino acids and predicts a molecular mass of 76.5 kDa. As a result of glycosylation, it migrates as an approximately 112.2 kDa band in SDS-PAGE under reducing conditions. -
Amino acids
1 to 685 -
Modifications
mutated Q677H -
Tags
His tag C-Terminus -
Additional sequence information
YP_009724390.1
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Specifications
Our Abpromise guarantee covers the use of ab289629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 89% PBS, 5% Mannitol, 5% Trehalose, 0.01% Tween 80
The formulation is lot specific. Please contact our Technical Support team for details.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionLot specific
Images
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SDS-PAGE - Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (mutated Q677H) (Active) (ab289629)SDS PAGE analysis of ab289629
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ELISA - Recombinant SARS-CoV-2 Spike Glycoprotein S1 (mutated Q677H) (ab289629)Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 µg/mL (100 µL/well) can bind ab289629, the EC50 of ab289629 is 100-600 ng/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab289629 has not yet been referenced specifically in any publications.
