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Recombinant human CX3CL1 protein (ab9820)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 97% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human CX3CL1 protein
    See all CX3CL1 proteins and peptides

  • Biological activity

    Determined by its ability to chemoattract human T cells using a concentration range of 5.0-10.0 ng/ml

  • Purity

    > 97 % SDS-PAGE.
    Sterile filtered Greater than 97% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      42 kDa

Specifications

Our Abpromise guarantee covers the use of ab9820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    The biological activity of this product is determined by its ability to chemoattract human T-lymphocytes using a concentration range of 5.0-10.0 ng/ml.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    For lot specific reconstitution information please contact our Scientific Support Team.

General Info

  • Alternative names

    • A 152E5.2
    • AB030188
    • ABCD 3
    • ABCD3
    • AI848747
    • C-X3-C motif chemokine 1
    • C3Xkine
    • Chemokine (C-X3-C motif) ligand 1
    • Chemokine C X3 C motif ligand 1
    • Chemokine CX3C Motif Ligand 1
    • CX3C membrane anchored chemokine
    • CX3C membrane-anchored chemokine
    • Cx3cl1
    • CXC 3
    • CXC3
    • CXC3C
    • D8Bwg0439e
    • FKN
    • Fractalkine
    • Neurotactin
    • NTN
    • NTT
    • Processed fractalkine
    • SCYD 1
    • SCYD1
    • Small inducible cytokine D1
    • Small inducible cytokine subfamily D (Cys X3 Cys) member 1
    • small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)
    • Small inducible cytokine subfamily D member 1
    • Small-inducible cytokine D1
    • X3CL1_HUMAN
    see all

  • Function

    The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

  • Tissue specificity

    Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas.

  • Sequence similarities

    Belongs to the intercrine delta family.

  • Post-translational
    modifications

    A soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
    O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab9820? Please let us know so that we can cite the reference in this datasheet.

ab9820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

A customer has just received the CX3CL1 protein #AB9820 and asked how to dissolve the lyophilized product. There are no recommendations on the datasheet. Because some proteins have to be solved not in water but in buffers or acids, I have not answered directly. I would appreciate your fast response. Kind regards,

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Answer

Thank you for your enquiry. The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20°C. We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. Aliquot and store at -20°C or -80°C.Avoid repeated freeze / thaw cycles.

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