Recombinant Human EEF2/Elongation factor 2 protein (denatured) (ab183170)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human EEF2/Elongation factor 2 protein (denatured) -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSDPVVSYRETVSEESNVLCLSKSPNKHN RLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIW CFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGV RFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQ CPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADL RSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPA LDNFLDKL -
Predicted molecular weight
34 kDa including tags -
Amino acids
574 to 858 -
Tags
His tag N-Terminus -
Additional sequence information
NP_001952
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab183170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as EEF2
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 2.4% Urea
General Info
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Alternative names
- EEF 2
- Eef2
- EF-2
see all -
Function
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. -
Sequence similarities
Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. -
Post-translational
modificationsPhosphorylation by EF-2 kinase completely inactivates EF-2.
Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis.
ISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human EEF2/Elongation factor 2 protein (denatured) (ab183170)
15% SDS-PAGE analysis of ab183170 (3μg).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183170 has not yet been referenced specifically in any publications.