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    products/proteins-peptides/recombinant-human-entactinnid-protein-ab158997.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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Recombinant Human Entactin/NID protein (ab158997)

  • Datasheet
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SDS-PAGE - Recombinant Human Entactin/NID protein (ab158997)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, WB

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    Description

    • Product name

      Recombinant Human Entactin/NID protein
      See all Entactin/NID proteins and peptides
    • Expression system

      Wheat germ
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        EGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYG ITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIERK
      • Amino acids

        1148 to 1247
      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab158997 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

    • Form

      Liquid
    • Additional notes

      This product was previously labelled as Entactin.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Enactin
      • Ent
      • Entactin
      • Entactin 1
      • NID
      • NID 1
      • NID-1
      • Nid1
      • NID1_HUMAN
      • Nidogen
      • Nidogen (enactin)
      • Nidogen 1
      • Nidogen-1
      see all
    • Function

      Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
    • Sequence similarities

      Contains 6 EGF-like domains.
      Contains 4 LDL-receptor class B repeats.
      Contains 1 NIDO domain.
      Contains 1 nidogen G2 beta-barrel domain.
      Contains 1 thyroglobulin type-1 domain.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Target information above from: UniProt accession P14543 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Entactin/NID protein (ab158997)
      SDS-PAGE - Recombinant Human Entactin/NID protein (ab158997)
      ab158997 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab158997? Please let us know so that we can cite the reference in this datasheet.

    ab158997 has not yet been referenced specifically in any publications.

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