Recombinant human Eph receptor A1/EphA1 protein (ab101778)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies
Description
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Product name
Recombinant human Eph receptor A1/EphA1 protein
See all Eph receptor A1/EphA1 proteins and peptides -
Biological activity
The Specific activity of ab101778 was determined to be 28 nmol/min/mg.
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Purity
> 90 % Densitometry.
Purity was determined to be >90% by densitometry. Affinity purified. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
72 kDa including tags -
Amino acids
569 to 976
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab101778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
Previously labelled as Eph receptor A1.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- EPH
- EPH receptor A1
- EPH tyrosine kinase
see all -
Function
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. -
Tissue specificity
Overexpressed in several carcinomas. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsPhosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant human Eph receptor A1/EphA1 protein (ab101778)SDS-PAGE showing ab101778 at approximately 72kDa.
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Functional Studies - Recombinant human Eph receptor A1/EphA1 protein (ab101778)The Specific activity of ab101778 was determined to be 28 nmol/min/mg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab101778 has not yet been referenced specifically in any publications.