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    products/proteins-peptides/recombinant-human-erk2-protein-ab43625.pdf

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Neuroscience Neurotransmission Intracellular Signaling Kinases
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Recombinant Human ERK2 protein (ab43625)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human ERK2 protein (ab43625) (ab43625)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% Densitometry
    • Suitable for: Functional Studies, WB

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    Description

    • Product name

      Recombinant Human ERK2 protein
      See all ERK2 proteins and peptides
    • Purity

      > 90 % Densitometry.

    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human

    Associated products

    • Related Products

      • Anti-ERK2 antibody (ab15282)
      • Anti-ERK2 antibody [E460] (ab32081)

    Specifications

    Our Abpromise guarantee covers the use of ab43625 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    General Info

    • Alternative names

      • ERK 2
      • ERK-2
      • ERT1
      • Extracellular Signal Regulated Kinase 2
      • Extracellular signal-regulated kinase 2
      • MAP kinase 1
      • MAP kinase 2
      • MAP kinase isoform p42
      • MAPK 1
      • MAPK 2
      • Mapk1
      • MAPK2
      • Mitogen-activated protein kinase 1
      • Mitogen-activated protein kinase 2
      • MK01_HUMAN
      • P38
      • P40
      • P41
      • p42-MAPK
      • P42MAPK
      • PRKM1
      • PRKM2
      • protein kinase, mitogen-activated, 1
      • protein kinase, mitogen-activated, 2
      • protein tyrosine kinase ERK2
      see all
    • Function

      Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
      Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain

      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications

      Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P28482 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human ERK2 protein (ab43625) (ab43625)
      SDS-PAGE - Recombinant Human ERK2 protein (ab43625) (ab43625)
      The purity of ab43625 was determined to be >90% by SDS-PAGE. MW ~42kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab43625? Please let us know so that we can cite the reference in this datasheet.

    ab43625 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    phone call about activity of this protein

    Read More

    Abcam community

    Verified customer

    Asked on Apr 02 2012

    Answer

    Vielen Dank für Ihren Anruf.

    Das Protein im Röhrchen selber wurde nach der Produktion in E. coli nicht aktiviert. Das Labor hat jedoch erfolgreich die Aktivität dieses Proteins demonstriert nach Aktivierung desselben mit MEK kinase.

    Ich hoffe diese Information ist hilfreich. Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    Answered on Apr 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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