Recombinant human ERK5 protein (ab126913)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: Functional Studies, WB, SDS-PAGE
Description
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Product name
Recombinant human ERK5 protein -
Biological activity
The specific activity of ab126913 was determined to be 11 nmol/min/mg
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Purity
> 75 % Densitometry.
Purity was determined to be >75% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
132 kDa including tags -
Amino acids
1 to 816
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab126913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Western blot
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Big MAP kinase 1
- BMK 1
- BMK 1 kinase
see all -
Function
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. -
Tissue specificity
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. -
Cellular localization
Cytoplasm. Nucleus. Translocates to the nucleus upon activation. - Information by UniProt
Images
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Functional Studies - Recombinant human ERK5 protein (ab126913)The specific activity of ERK5 (ab126913) was determined to be 2.5 nmol/min/mg as per activity assay protocol and was equivalent to 12.7 nmol/min/mg as per radiometric assay
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SDS-PAGE - Recombinant human ERK5 protein (ab126913)SDS PAGE analysis of ab126913 -
SDS-PAGE - Recombinant human ERK5 protein (ab126913)SDS PAGE analysis of ab126913 -
SDS-PAGE - Recombinant human ERK5 protein (ab126913)SDS-PAGE using ab126913 -
Functional Studies - Recombinant human ERK5 protein (ab126913)The specific activity of ab126913 was determined to be 11 nmol/min/mg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126913 has been referenced in 1 publication.
- Liu W et al. Metabolic stress-induced cardiomyopathy is caused by mitochondrial dysfunction due to attenuated Erk5 signaling. Nat Commun 8:494 (2017). PubMed: 28887535