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    products/proteins-peptides/recombinant-human-gpsn2-pkn1-fusion-protein-tagged-ab269032.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases S6K Family
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Recombinant human GPSN2-PKN1 fusion protein (Tagged) (ab269032)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)
  • SDS-PAGE - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human GPSN2-PKN1 fusion protein (Tagged)
  • Biological activity

    Recombinant human PKN1 + GPSN2 fusion protein activity was determined to be 245.5 nmol/min/mg in a kinase assay using CREBtide synthetic peptide substrate.

  • Purity

    > 90 % SDS-PAGE.
    Affinity purified.
  • Expression system

    Baculovirus infected Sf9 cells
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      GPSN2: aa1-5 (exon1) PKN1: aa 473-end (exons 10-22)
    • Molecular weight information

      Approx 80 kDa by SDS-PAGE.
    • Amino acids

      1 to 5
    • Tags

      GST tag N-Terminus
    • Additional sequence information

      NM_138501 NM_002741
  • Description

    Recombinant human PKN1 + GPSN2 protein (Active)

Specifications

Our Abpromise guarantee covers the use of ab269032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    GST-GPSN2-PKN1 fusion protein

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Cellular localization

    PKN1: Cytoplasm. Nucleus. Endosome. Cell membrane. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. GPSN2: Endoplasmic reticulum membrane; Multi-pass membrane protein.

Images

  • Functional Studies - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)
    Functional Studies - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)

    Recombinant human PKN1 + GPSN2 fusion protein activity was determined to be 245.5 nmol/min/mg in a kinase assay using CREBtide synthetic peptide substrate.

  • SDS-PAGE - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)
    SDS-PAGE - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)

    Recombinant human TECR-PKN1 fusion protein (ab269032) resolved on a SDS-PAGE gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab269032? Please let us know so that we can cite the reference in this datasheet.

ab269032 has not yet been referenced specifically in any publications.

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