Recombinant human GPSN2-PKN1 fusion protein (Tagged) (ab269032)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human GPSN2-PKN1 fusion protein (Tagged) -
Biological activity
Recombinant human PKN1 + GPSN2 fusion protein activity was determined to be 245.5 nmol/min/mg in a kinase assay using CREBtide synthetic peptide substrate.
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Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GPSN2: aa1-5 (exon1) PKN1: aa 473-end (exons 10-22) -
Molecular weight information
Approx 80 kDa by SDS-PAGE. -
Amino acids
1 to 5 -
Tags
GST tag N-Terminus -
Additional sequence information
NM_138501 NM_002741
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Description
Recombinant human PKN1 + GPSN2 protein (Active)
Specifications
Our Abpromise guarantee covers the use of ab269032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
GST-GPSN2-PKN1 fusion protein
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Cellular localization
PKN1: Cytoplasm. Nucleus. Endosome. Cell membrane. Cleavage furrow. Midbody. Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-374 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. GPSN2: Endoplasmic reticulum membrane; Multi-pass membrane protein.
Images
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Functional Studies - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)
Recombinant human PKN1 + GPSN2 fusion protein activity was determined to be 245.5 nmol/min/mg in a kinase assay using CREBtide synthetic peptide substrate.
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SDS-PAGE - Recombinant human PKN1 + GPSN2 protein (Tagged) (ab269032)Recombinant human TECR-PKN1 fusion protein (ab269032) resolved on a SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab269032 has not yet been referenced specifically in any publications.