Recombinant human Growth Hormone protein (Active) (ab280333)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, MS, HPLC, Functional Studies, Sandwich ELISA
Description
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Product name
Recombinant human Growth Hormone protein (Active)
See all Growth Hormone proteins and peptides -
Biological activity
Fully biologically active determined by the dose dependent cell proliferation of rat Nb2-11 lymphoma cell line.
ED50 is ≤ 0.21 ng/ml, corresponding to a specific activity of 4.76 x 106 units/mg.
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Purity
>= 95 % SDS-PAGE.
=95% Purity by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F -
Predicted molecular weight
22 kDa -
Actual molecular weight
22 kDa -
Molecular weight information
Predicted MW is 22186.1 Da (+/- 10 Da by ESI-TOF). Observed mass is 22187.15 -
Amino acids
27 to 217 -
Additional sequence information
N-terminal glycine. Full-length mature chain lacking the signal peptde.
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Specifications
Our Abpromise guarantee covers the use of ab280333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
HPLC
Functional Studies
Sandwich ELISA
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- gH
- GH-N
- GH1
see all -
Function
Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. -
Involvement in disease
Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. -
Sequence similarities
Belongs to the somatotropin/prolactin family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Biological Activity - Recombinant human Growth Hormone protein (Active) (ab280333)
Fully biologically active determined by the dose dependent cell proliferation of rat Nb2-11 lymphoma cell line.
ED50 is ≤ 0.21 ng/ml, corresponding to a specific activity of 4.76 x 106 units/mg.
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SDS-PAGE - Recombinant human Growth Hormone protein (Active) (ab280333)SDS-PAGE analysis of ab280333.
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Sandwich ELISA - Recombinant human Growth Hormone protein (Active) (ab280333)Sandwich ELISA - Recombinant human Growth Hormone protein standard curve.
Background subtracted standard curve using Human Growth Hormone Antibody Pair - BSA and Azide free (ab241146) and Recombinant human Growth Hormone protein (ab280333) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
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Mass Spectrometry - Recombinant human Growth Hormone protein (Active) (ab280333)Mass determination by ESI-TOF.
Predicted MW is 22186.1 Da (+/- 10 Da by ESI-TOF). Observed mass is 22187.15
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HPLC - Recombinant human Growth Hormone protein (Active) (ab280333)HPLC analysis of ab280333.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab280333 has not yet been referenced specifically in any publications.
