Recombinant human GST-EML4-ALK fusion protein (Active) (ab268525)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human GST-EML4-ALK fusion protein (Active) -
Biological activity
The specific activity of ab268525 was 15 nmol/min/mg in a peptide kinase assay using IGF1Rtide (KKKSPGEYVNIEFG) as substrate.
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Purity
> 70 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MDGFAGSLDD SISAASTSDV QDRLSALESR VQQQEDEITV LKAALADVLR RLAISEDHVA SVKKSVSSKG QPSPRAVIPM SCITNGSGAN RKPSHTSAVS IAGKETLSSA AKSGTEKKKE KPQGQREKKE ESHSNDQSPQ IRASPSPQPS SQPLQIHRQT PESKNATPTK SIKRPSPAEK SHNSWENSDD SRNKLSKIPS TPKLIPKVTK TADKHKDVII NQ -
Molecular weight information
Approx 118kDa by SDS-PAGE (GST-EML4-AK fusion protein) -
Amino acids
1 to 222 -
Tags
GST tag N-Terminus -
Additional sequence information
GenBank: NM_019063
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Amino Acid Sequence 2
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Species
Human -
Sequence
VYRRKHQELQ AMQMELQSPE YKLSKLRTST IMTDYNPNYC FAGKTSSISD LKEVPRKNIT LIRGLGHGAF GEVYEGQVSG MPNDPSPLQV AVKTLPEVCS EQDELDFLME ALIISKFNHQ NIVRCIGVSL QSLPRFILLE LMAGGDLKSF LRETRPRPSQ PSSLAMLDLL HVARDIACGC QYLEENHFIH RDIAARNCLL TCPGPGRVAK IGDFGMARDI YRASYYRKGG CAMLPVKWMP PEAFMEGIFT SKTDTWSFGV LLWEIFSLGY MPYPSKSNQE VLEFVTSGGR MDPPKNCPGP VYRIMTQCWQ HQPEDRPNFA IILERIEYCT QDPDVINTAL PIEYGPLVEE EEKVPVRPKD PEGVPPLLVS QQAKREEERS PAAPPPLPTT SSGKAAKKPT AAEISVRVPR GPAVEGGHVN MAFSQSNPPS ELHKVHGSRN KPTSLWNPTY GSWFTEKPTK KNNPIAKKEP HDRGNLGLEG SCTVPPNVAT GRLPGASLLL EPSSLTANMK EVPLFRLRHF PCGNVNYGYQ QQGLPLEAAT APGAGHYEDT ILKSKNSMNQ PGP -
Molecular weight information
Approx 118 kDa by SDS-PAGE (GST-EML4-ALK fusion protein) -
Amino acids
1058 to 1620 -
Additional sequence information
GenBank: NM_004304
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Description
Recombinant human ALK + EML4 protein (Active)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab268525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
GST-EML4-ALK fusion protein
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Cellular localization
ALK: Cell membrane. Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. EML4: Cytoplasm > cytoskeleton.
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab268525 has not yet been referenced specifically in any publications.