Recombinant human HDAC1 protein (ab101661)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, WB
Description
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Product name
Recombinant human HDAC1 protein
See all HDAC1 proteins and peptides -
Biological activity
The Specific activity of ab101661 was determined to be 8900 RLU/min/ng.
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Purity
> 70 % SDS-PAGE.
>70% by densitometry. Affinity purified. -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
88 kDa including tags -
Amino acids
1 to 482
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab101661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DKFZp686H12203
- GON 10
- HD1
see all -
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
Tissue specificity
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
Images
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Functional Studies - Recombinant human HDAC1 protein (ab101661)The specific activity of HDAC1 (ab101661) was determined to be 10230 RLU/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human HDAC1 protein (ab101661)SDS PAGE analysis of ab101661 -
SDS-PAGE - Recombinant human HDAC1 protein (ab101661)SDS PAGE analysis of ab101661 -
SDS-PAGE - Recombinant human HDAC1 protein (ab101661)SDS-PAGE showing ab101661 at approximately 88kDa. -
Functional Studies - Recombinant human HDAC1 protein (ab101661)The Specific activity of ab101661 was determined to be 8900 RLU/min/ng.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab101661 has been referenced in 4 publications.
- Ahuja G et al. Loss of genomic integrity induced by lysosphingolipid imbalance drives ageing in the heart. EMBO Rep 20:N/A (2019). PubMed: 30886000
- Huang Y et al. Discovery of Janus Kinase 2 (JAK2) and Histone Deacetylase (HDAC) Dual Inhibitors as a Novel Strategy for the Combinational Treatment of Leukemia and Invasive Fungal Infections. J Med Chem 61:6056-6074 (2018). PubMed: 29940115
- Zhang Y et al. Discovery of a fluorescent probe with HDAC6 selective inhibition. Eur J Med Chem 141:596-602 (2017). PubMed: 29102179
- Duan W et al. Design, synthesis, and antitumor evaluation of novel histone deacetylase inhibitors equipped with a phenylsulfonylfuroxan module as a nitric oxide donor. J Med Chem 58:4325-38 (2015). PubMed: 25906087