Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag N-Terminus, DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human Hemoglobin subunit alpha protein (denatured)
See all Hemoglobin subunit alpha proteins and peptides -
Purity
> 85 % SDS-PAGE.
ab131697 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMVL SPADKTNVKA AWGKVGAHAG EYGAEALERM FLSFPTTKTY FPHFDLSHGS AQVKGHGKKV ADALTNAVAH VDDMPNALSA LSDLHAHKLR VDPVNFKLLS HCLLVTLAAH LPAEFTPAVH ASLDKFLASV STVLTSKYR -
Predicted molecular weight
20 kDa including tags -
Amino acids
1 to 142 -
Tags
His tag N-Terminus , DDDDK tag N-Terminus
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Associated products
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Related Products
- Anti-Hemoglobin subunit zeta + Hemoglobin subunit alpha antibody [EP3607] (ab109444)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [F-tag-01] (ab18230)
- Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab21536)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)
Specifications
Our Abpromise guarantee covers the use of ab131697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 12.01% Urea, 0.03% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
General Info
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Alternative names
- Alpha 1 globin
- Alpha globin
- Alpha one globin
see all -
Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Hemoglobin subunit alpha protein (denatured) (ab131697)
SDS-PAGE analysis of ab131697 (3 µg) under reducing conditions and visualized by coomassie blue stain.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131697 has not yet been referenced specifically in any publications.