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Recombinant human Hexokinase II protein (His tag) (ab85920)

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SDS-PAGE - Recombinant human Hexokinase II protein (His tag) (ab85920)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: Functional Studies, SDS-PAGE

    Description

    • Product name

      Recombinant human Hexokinase II protein (His tag)
      See all Hexokinase II proteins and peptides

    • Biological activity

      Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C. Specific activity is 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30C.

    • Purity

      > 95 % SDS-PAGE.
      ab85920 is prified using conventional chromatography techniques.

    • Expression system

      Escherichia coli

    • Accession

      P52789

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR

      • Predicted molecular weight

        102 kDa

      • Amino acids

        1 to 917

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab85920 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • DKFZp686M1669
      • Hexokinase 2
      • Hexokinase 2 muscle
      • Hexokinase type II
      • Hexokinase-2
      • HK 2
      • HK II
      • HK2
      • HKII
      • HxK 2
      • HxK2
      • HXK2_HUMAN
      • Muscle form hexokinase
      see all

    • Tissue specificity

      Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

    • Pathway

      Carbohydrate metabolism; hexose metabolism.

    • Sequence similarities

      Belongs to the hexokinase family.
      Contains 2 hexokinase domains.

    • Domain

      The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.

    • Cellular localization

      Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant human Hexokinase II protein (His tag) (ab85920)
      SDS-PAGE - Recombinant human Hexokinase II protein (His tag) (ab85920)
      12% SDS-PAGE showing ab85920 at approximately 104.1kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab85920? Please let us know so that we can cite the reference in this datasheet.

    ab85920 has not yet been referenced specifically in any publications.

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