Recombinant Human Histone H4 + Histone H3 protein Tetramer (His tag) (ab278809)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 80% SDS-PAGE
- Tags: His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE
Description
-
Product name
Recombinant Human Histone H4 + Histone H3 protein Tetramer (His tag) -
Purity
>= 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
-
Amino Acid Sequence 1
-
Species
Human -
Sequence
MARTKQTARK STGGKAPRKQ LATKAARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSSAV MALQEACEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA -
Predicted molecular weight
57 kDa including tags -
Molecular weight information
Predicted mass of tetramer -
Amino acids
1 to 136 -
Tags
His tag N-Terminus
-
Amino Acid Sequence 2
-
Species
Human -
Sequence
MSGRGKGGKG LGKGGAKRHR KVLRDNIQGI TKPAIRRLAR RGGVKRISGL IYEETRGVLK VFLENVIRDA VTYTEHAKRK TVTAMDVVYA LKRQGRTLYG FGG -
Predicted molecular weight
57 kDa including tags -
Molecular weight information
Predicted MW of tetramer. -
Amino acids
1 to 103 -
Tags
His tag N-Terminus
-
-
Description
Recombinant Human Histone H4 + Histone H3 protein (His tag)
Specifications
Our Abpromise guarantee covers the use of ab278809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
-
Form
Liquid -
Additional notes
Human recombinant histone tetramer, full length, consisting of 2 molecules each of histones H3 (a.a. 1-136(end)), and H4 (a.a. 1-103(end)), each with an N-terminal His-tag, expressed in an E. coli expression system.
Suitable as a substrate for Histone Methyltransferases.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 10% Glycerol (glycerin, glycerine), 11.7% Sodium chloride, 0.015% DTT, 0.04% EDTA disodium salt, 0.7% Tris HCl
General Info
-
Cellular localization
Histone H4: Nucleus. Chromosome. Histone H3: Nucleus. Chromosome.
Images
-
Western blot - Recombinant Human Histone H4 + Histone H3 protein Tetramer (His tag) (ab278809)
SDS-PAGE analysis of ab278809 (2 μg).
4-20% SDS-PAGE. Coomassie staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab278809 has not yet been referenced specifically in any publications.