Recombinant Human HIV-1 gp120 (CN54) protein (ab174070)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Human HIV-1 gp120 (CN54) protein -
Biological activity
Immobilized Human CD4 at 2 μg/mL (100 μL/well) can bind HIV-1 GP120 (ab174070), His Tag with a linear range of 0.5-63 ng/mL
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Purity
> 95 % SDS-PAGE.
Purified by Immobilized metal affinity chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
TVYYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVL ENVTENFNMWKNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLECRQVNT TNATSSVNVTNGEEIKNCSFNATTEIRDKKQKVYALFYRLDIVPLEEERK GNSSKYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNG TGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTII VHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGNIRQAYCNIKKD DWIRTLQRVGKKLAEHFPRRIINFTSPAGGDLEITTHSFNCRGEFFYCNT SSLFNSTYNPNDTNSNSSSSNSSLDITIPCRIKQIINMWQEVGRAMYAPP IEGNITCKSNITGLLLVRDGGVESNETEIFRPGGGDMRNNWRSELYKYKV VEIKPLGIAPTAAKRRVVEREKR -
Predicted molecular weight
54 kDa including tags -
Amino acids
34 to 506 -
Tags
His tag C-Terminus
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Description
Recombinant human HIV-1 gp120 (CN54) protein
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- Envelope glycoprotein gp160
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Relevance
The envelope glyprotein gp160 precursor down-modulates cell surface CD4 antigen by interacting with it in the endoplasmic reticulum and blocking its transport to the cell surface By similarity. The gp120-gp41 heterodimer allows rapid transcytosis of the virus through CD4 negative cells such as simple epithelial monolayers of the intestinal, rectal and endocervical epithelial barriers. Both gp120 and gp41 specifically recognize glycosphingolipids galactosyl-ceramide (GalCer) or 3' sulfo-galactosyl-ceramide (GalS) present in the lipid rafts structures of epithelial cells. Binding to these alternative receptors allows the rapid transcytosis of the virus through the epithelial cells. This transcytotic vesicle-mediated transport of virions from the apical side to the basolateral side of the epithelial cells does not involve infection of the cells themselves.
Images
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SDS-PAGE - Recombinant Human HIV-1 gp120 (CN54) protein (ab174070)
SDS-PAGE analysis of reduced ab174070 stained overnight with Coomassie Blue.
DTT-reduced Protein migrates as 65-110 kDa due to glycosylation.
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Functional Studies - Recombinant human HIV-1 gp120 (CN54) protein (ab174070)Immobilized Human CD4, Fc Tag at 2 μg/mL (100 μL/well) can bind ab174070 with a linear range of 0.5-63 ng/mL (QC tested).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab174070 has been referenced in 1 publication.
- Seiler BT et al. Broad-spectrum capture of clinical pathogens using engineered Fc-mannose-binding lectin enhanced by antibiotic treatment. F1000Res 8:108 (2019). PubMed: 31275563
