Recombinant human Hsp90 beta protein (Active) (ab80033)
Key features and details
- Expression system: Baculovirus infected BTI-TN-5B1-4 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, ELISA, Inhibition Assay, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human Hsp90 beta protein (Active)
See all Hsp90 beta proteins and peptides -
Biological activity
1. The reactions are in 33 mM Hepes pH7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37ºC. It is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.
2. The protein concentrations (where applicable) were 2 µM Hsp90 (Hsp90 beta), 2 µM Ahsa1 (His tagged human Ahsa1), 4 µM p23 (cleaved human p23), 4 µM HOP (His-tagged human HOP).
3. Addition of a two-fold excess of p23 reduced Aha1-mediated Hsp90 stimulation by 30% (when using 2 µM Hsp90 and Aha1).
4. Addition of a two -fold excess of HOP completely eliminated Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity.
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Purity
> 90 % SDS-PAGE.
ab80033 is Affinity Purified, Endotoxin-free -
Expression system
Baculovirus infected BTI-TN-5B1-4 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDA LDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLIN NLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHN DDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKE VVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKI EDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEE YGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKK NNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKI LKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRR LSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFV ERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEK KKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTA NMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKA VKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEP NAAVPDEIPPLEGDEDASRMEEVD -
Predicted molecular weight
90 kDa -
Amino acids
1 to 724
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab80033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
Inhibition Assay
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab80033 is endotoxin free. <0.1 EU/ml. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 0.0154% DTT, 0.242% Tris, 0.00292% EDTA, 10% Glycerol (glycerin, glycerine), 1.015% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 90 kda heat shock protein beta HSP90 beta
- D6S182
- FLJ26984
see all -
Function
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Domain
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. -
Post-translational
modificationsUbiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Images
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Western blot - Recombinant human Hsp90 beta protein (ab80033)
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SDS-PAGE - Recombinant human Hsp90 beta protein (Active) (ab80033)SDS-PAGE of Hsp90 beta protein (ab80033).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab80033 has been referenced in 1 publication.
- Liu S et al. A method to measure the denatured proteins in the corona of nanoparticles based on the specific adsorption of Hsp90ab1. Nanoscale 12:15857-15868 (2020). PubMed: 32696774
