Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human IgG1 protein (Active)
See all IgG1 proteins and peptides
Immobilized Human CD64, His Tag at 1 μg/mL (100 μL/well) can bind Recombinant Human IgG1 protein (ab155632) with a linear range of 1-16 ng/mL.
Purity> 95 % SDS-PAGE.
Endotoxin level< 1.000 Eu/µg
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLN GKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSL TCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKS RWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Predicted molecular weight26 kDa
Amino acids99 to 330
Our Abpromise guarantee covers the use of ab155632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Upon reconstitution ab155632 should be aliquoted and stored at -80°C and is stable for 3 months.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
Constituents: 0.6057% Tris, 0.7507% Glycine, 0.05844% Sodium chloride, 12% D-(+)-Trehalose dihydrate
Normally trehalose is added as protectant
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Ig gamma 1 chain C region
- IgG heavy chain locus
RelevanceThere are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
Cellular localizationSecreted. Cell membrane; Single-pass membrane protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab155632 has not yet been referenced specifically in any publications.