Recombinant human IgG1 protein (Active) (ab155632)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human IgG1 protein (Active)
See all IgG1 proteins and peptides -
Biological activity
Immobilized Human CD64, His Tag at 1 μg/mL (100 μL/well) can bind Recombinant Human IgG1 protein (ab155632) with a linear range of 1-16 ng/mL.
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Purity
> 95 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
EPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLN GKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSL TCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKS RWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
Predicted molecular weight
26 kDa -
Amino acids
99 to 330
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Specifications
Our Abpromise guarantee covers the use of ab155632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
Upon reconstitution ab155632 should be aliquoted and stored at -80°C and is stable for 3 months.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7.50
Constituents: 0.6057% Tris, 0.7507% Glycine, 0.05844% Sodium chloride, 12% D-(+)-Trehalose dihydrate
Normally trehalose is added as protectantThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- Ig gamma 1 chain C region
- IgG heavy chain locus
- IGHG1
see all -
Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
Cellular localization
Secreted. Cell membrane; Single-pass membrane protein
Images
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SDS-PAGE - Recombinant Human IgG1 protein (ab155632)
Recombinant Tag-free Human IgG1 protein on SDS-PAGE under reducing conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human IgG1 protein (Active) (ab155632)Immobilized Human CD64, His Tag at 1 μg/mL (100 μL/well) can bind ab155632 with a linear range of 1-16 ng/mL (QC tested).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab155632 has not yet been referenced specifically in any publications.
