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    products/proteins-peptides/recombinant-human-igj-protein-ab140727.pdf

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Immunology Immunoglobulins Heavy Chain IgA
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Recombinant Human IGJ protein (ab140727)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human IGJ protein (ab140727)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

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    Description

    • Product name

      Recombinant Human IGJ protein
      See all IGJ proteins and peptides
    • Purity

      > 85 % SDS-PAGE.
      ab140727 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      P01591
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHHSSGLVPRGSHMGSQEDERIVLVDNKCKCARITSRIIRSSE DPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEV ELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVET ALTPDACYPD
      • Predicted molecular weight

        18 kDa including tags
      • Amino acids

        23 to 159
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-IGJ antibody [SP105] (ab105229)
      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab140727 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • IGCJ
      • IGJ_HUMAN
      • Immunoglobulin J chain
      • Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides
      • immunoglobulin joining chain
      • JCH
      • JCHAIN
      • Joining chain of multimeric IgA and IgM
      see all
    • Function

      Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01591 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human IGJ protein (ab140727)
      SDS-PAGE - Recombinant Human IGJ protein (ab140727)
      15% SDS-PAGE analysis of ab140727 (3µg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab140727? Please let us know so that we can cite the reference in this datasheet.

    ab140727 has been referenced in 1 publication.

    • Byron MJ  et al. Immunoglobulin J chain as a non-invasive indicator of pregnancy in the cheetah (Acinonyx jubatus). PLoS One 15:e0225354 (2020). PubMed: 32040477

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