Recombinant Human IL-13 protein (Active) (ab270079)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, MS, Functional Studies
Description
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Product name
Recombinant Human IL-13 protein (Active)
See all IL-13 proteins and peptides -
Biological activity
Fully biologically active determined bydose dependent proliferation of TF-1 cells.
ED50 is ≤ 18.99 ng/ml, corresponding to a specific activity of 5.3 x 104 units/mg.
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Purity
>= 95 % SDS-PAGE.
=95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
LTCLGGFASP GPVPPSTALR ELIEELVNIT QNQKAPLCNG SMVWSINLTA GMYCAALESL INVSGCSAIE KTQRMLSGFC PHKVSAGQFS SLHVRDTKIE VAQFVKDLLL HLKKLFREGR FN -
Predicted molecular weight
13 kDa -
Actual molecular weight
13 kDa -
Molecular weight information
Predicted MW is 13348.61 Da (+/- 10 Da by ESI-TOF). Observed MW is13352.33 Da. -
Amino acids
25 to 146 -
Additional sequence information
N-terminal glycine Full-length mature chain lacking the signal peptide.
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Description
Recombinant human IL-13 protein
Specifications
Our Abpromise guarantee covers the use of ab270079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
SDS-PAGE
Mass Spectrometry
Functional Studies
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Form
Lyophilized -
Additional notes
For research use only and are not intended for diagnostic or therapeutic use, not for use in humans.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- Allergic rhinitis
- ALRH
- BHR 1
see all -
Function
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
Involvement in disease
Allergic rhinitis -
Sequence similarities
Belongs to the IL-4/IL-13 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-13 protein (Active) (ab270079)
Fully biologically active determined bydose dependent proliferation of TF-1 cells.
ED50 is ≤ 18.99 ng/ml, corresponding to a specific activity of 5.3 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3383878-6.
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SDS-PAGE - Recombinant Human IL-13 protein (ab270079)SDS-PAGE analysis of ab270079.
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Mass Spectrometry - Recombinant Human IL-13 protein (ab270079)Mass determination by ESI-TOF.
Predicted MW is 13348.61 Da. (+/- 10 Da by ESI-TOF). Observed MW is 13352.33 Da.
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HPLC - Recombinant Human IL-13 protein (ab270079)HPLC analysis of ab270079.
Peak at 4 min due to deglycosylation reaction mixture.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab270079 has not yet been referenced specifically in any publications.
