Recombinant human IL-21 protein (Animal Free) (ab179621)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human IL-21 protein (Animal Free)
See all IL-21 proteins and peptides -
Biological activity
B9 cell proliferation: ED50 ≤50ng/ml; ≥ 2.0 x 104 units/mg.
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Purity
> 95 % SDS-PAGE.
The purity of ab179621 was determined using reducing and non-reducing SDS-PAGE, UV spectroscopy at 280 nm. -
Endotoxin level
<=1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS -
Predicted molecular weight
16 kDa -
Amino acids
25 to 155 -
Additional sequence information
Mature form.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab179621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituent: 0.28% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CVID11
- IL 21
- IL-21
see all -
Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-21 protein (Animal Free) (ab179621)Functional analysis of ab179621
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SDS-PAGE - Recombinant human IL-21 protein (Animal Free) (ab179621)SDS PAGE analysis of ab179621 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab179621 has not yet been referenced specifically in any publications.
