Recombinant human IL-22 protein (Active) (ab280331)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, MS, HPLC, Cell Culture
Description
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Product name
Recombinant human IL-22 protein (Active)
See all IL-22 proteins and peptides -
Biological activity
Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.
ED50 is 390 pg/ml.
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Purity
>= 95 % SDS-PAGE.
=95% Purity by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
APISSHCRLD KSNFQQPYIT NRTFMLAKEA SLADNNTDVR LIGEKLFHGV SMSERCYLMK QVLNFTLEEV LFPQSDRFQP YMQEVVPFLA RLSNRLSTCH IEGDDLHIQR NVQKLKDTVK KLGESGEIKA IGELDLLFMS LRNACI -
Predicted molecular weight
17 kDa -
Actual molecular weight
17 kDa -
Molecular weight information
Predicted MW is 16806.39 Da (+/- 10 Da by ESI-TOF). Observed MW is 16809.33 -
Amino acids
34 to 179 -
Additional sequence information
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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Specifications
Our Abpromise guarantee covers the use of ab280331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Mass Spectrometry
HPLC
Cell Culture
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- Cytokine Zcyto18
- IL 10 related T cell derived inducible factor
- IL 21
see all -
Function
Cytokine that contributes to the inflammatory response in vivo. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-22 protein (ab280331)
Functional analysis of ab280331.
Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.
ED50 is 390 pg/ml.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3377996-1
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SDS-PAGE - Recombinant Human IL-22 protein (ab280331)SDS-PAGE analysis of ab280331.
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Mass Spectrometry - Recombinant Human IL-22 protein (ab280331)Mass determination by ESI-TOF.
Predicted MW is 16806.39 Da (+/- 10 Da by ESI-TOF). Observed MW is 16809.33
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HPLC - Recombinant Human IL-22 protein (ab280331)HPLC analysis of ab280331.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab280331 has not yet been referenced specifically in any publications.
