Recombinant human Lyn protein (ab70789)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
-
Product name
Recombinant human Lyn protein
See all Lyn proteins and peptides -
Biological activity
The Specific activity of ab70789 was determined to be 571 nmol/min/mg.
-
Purity
> 90 % SDS-PAGE.
Purity: was determined to be >90% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH DFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMGCI SKGKDSLSD DGVDLKTQPV RNTERTIYVR PTSNKQQRP VPESQLLPGQ RFQTKDPEEQ GDIVVALYPY DGIHPDDLSF KKGEKMKVLE EHGEWWKAKS LTKKEGFIP SNYVAKLNTL ETEEWFFKDI TRKDAERQLL PGNSAGAFL IRESETLKGS FSLSVRDFDP VHGDVIKHYK IRSLDNGGYY ISPRITFPCI SDMIKHYQKQ ADGLCRRLEK ACISPKPQKP WDKDAWEIPR ESIKLVKRLG AGQFGEVWMG YYNNSTKVAV KTLKPGTMSV QAFLEEANLM TLQHDKLVR LYAVVTREEP IYIITEYMAK GSLLDFLKSD EGGKVLLPKL IDFSAQIAEG MAYIERKNYI HRDLRAANVL VSESLMCKIA DFGLARVIED NEYTAREGAK FPIKWTAPEA INFGCFTIKS DVWSFGILLY EIVTYGKIPY PGRTNADVMT LSQGYRMPR VENCPDELYD IMKMCWKEKA ERPTFDYLQ SVLDDFYTAT EGQYQQQP -
Predicted molecular weight
85 kDa -
Tags
GST tag N-Terminus
-
Associated products
-
Related Products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab70789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
-
Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- AA407514
- EC 2.7.10.2
- FLJ26625
see all -
Function
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. -
Tissue specificity
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Domain
The protein kinase domain plays an important role in its localization in the cell membrane. -
Post-translational
modificationsUbiquitinated. Ubiquitination is SH3-dependent. -
Cellular localization
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. - Information by UniProt
Images
-
Functional Studies - Recombinant human Lyn protein (ab70789)The specific activity of Lyn (ab70789) was determined to be 485 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant human Lyn protein (ab70789)SDS PAGE analysis of ab70789 -
SDS-PAGE - Recombinant human Lyn protein (ab70789)SDS-PAGE showing ab70789 at approximately 85 kDa.
-
Functional Studies - Recombinant human Lyn protein (ab70789)Kinase Assay demonstrating specific activity of ab70789.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab70789 has not yet been referenced specifically in any publications.