Recombinant human MCP1 protein (Active) (ab270081)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Sandwich ELISA, Cell Culture, SDS-PAGE, Functional Studies, MS, HPLC
Description
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Product name
Recombinant human MCP1 protein (Active)
See all MCP1 proteins and peptides -
Biological activity
Fully biologically active determined by the dose dependent migration of human PBMCs or THP-1 monocytes.
ED50 is ≤10-50 ng/ml, corresponding to a specific activity of 0.1-0.02 x 106 units/mg.
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Purity
>= 95 % SDS-PAGE.
=95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT -
Predicted molecular weight
9 kDa -
Actual molecular weight
9 kDa -
Molecular weight information
Predicted mass is 8742.08 Da (+/- 10 Da by ESI TOF). Observed mass is 8742 Da. -
Amino acids
24 to 99 -
Additional sequence information
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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Specifications
Our Abpromise guarantee covers the use of ab270081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Sandwich ELISA
Cell Culture
SDS-PAGE
Functional Studies
Mass Spectrometry
HPLC
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see all -
Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human MCP1 protein (ab270081)
Fully biologically active determined by the dose dependent migration of human PBMCs or THP-1 monocytes.
ED50 is ≤10-50 ng/ml, corresponding to a specific activity of 0.1-0.02 x 106 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3368303-1
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SDS-PAGE - Recombinant human MCP1 protein (ab270081)SDS-PAGE analysis of ab270081.
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Mass Spectrometry - Recombinant human MCP1 protein (ab270081)Mass determination by ESI TOF.
Predicted mass is 8742.08 Da (+/- 10 Da by ESI TOF). Observed mass is 8742 Da.
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HPLC - Recombinant human MCP1 protein (ab270081)HPLC analysis of ab270081.
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Sandwich ELISA - Recombinant human MCP1 protein (Active) (ab270081)Background subtracted standard curve using Human MCP1 Antibody Pair - BSA and Azide free (ab241803) and Recombinant human MCP1 protein (Active) (ab270081) in sandwich ELISA.
The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab270081 has not yet been referenced specifically in any publications.