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Bioactive grade

Recombinant human MMP3 protein (Active) (ab96555)

Submit a review Q&A (3)References (3)
Western blot - Recombinant human MMP3 protein (ab96555)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: >= 98% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Active: Yes
    • Suitable for: HPLC, SDS-PAGE, Functional Studies, WB

    Description

    • Product name

      Recombinant human MMP3 protein (Active)
      See all MMP3 proteins and peptides

    • Biological activity

      ab96555 activity was measured by its ability to cleave a chromogenic peptide MMP3 substrate at room temperature.

      At a MMP3 concentration of 2.5 µg/ml, 50% cleavage was achieved at an incubation time of approximately 75 minutes.

    • Purity

      >= 98 % SDS-PAGE.
      >=98% HPLC analyses.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Escherichia coli

    • Accession

      P08254

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC

      • Predicted molecular weight

        43 kDa

      • Amino acids

        101 to 477

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab96555 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      HPLC

      SDS-PAGE

      Functional Studies

      Western blot

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..

      Constituents: 0.00111% Calcium chloride, 0.164% Sodium phosphate, 0.1% CHAPS, 0.002% EDTA disodium salt

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex.

    General Info

    • Alternative names

      • CHDS6
      • Matrix metalloproteinase 3
      • Matrix metalloproteinase 3 preproprotein
      • Matrix metalloproteinase-3
      • MGC126102
      • MGC126103
      • MGC126104
      • MMP 3
      • MMP-3
      • MMP3
      • MMP3_HUMAN
      • Proteoglycanase
      • SL-1
      • SL1
      • STMY
      • STMY1
      • STR1
      • Stromelisin 1
      • Stromelysin 1
      • Stromelysin 1 progelatinase
      • Stromelysin-1
      • Transin 1
      • Transin-1
      see all

    • Function

      Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.

    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    Images

    • Western blot - Recombinant human MMP3 protein (ab96555)
      Western blot - Recombinant human MMP3 protein (ab96555)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab96555? Please let us know so that we can cite the reference in this datasheet.

    ab96555 has been referenced in 3 publications.

    • Pentz R  et al. A new role for matrix metalloproteinase-3 in the NGF metabolic pathway: Proteolysis of mature NGF and sex-specific differences in the continuum of Alzheimer's pathology. Neurobiol Dis 148:105150 (2021). PubMed: 33130223
    • Ganguly SS  et al. Notch3 promotes prostate cancer-induced bone lesion development via MMP-3. Oncogene 39:204-218 (2020). PubMed: 31467432
    • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). PubMed: 28158775

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    When you did the test, did you incubate with trypsin first?

    Read More
    Answer

    The protocol for recombinant human MMP-3/MMP-2 bioactivity used is as follows:

    Kit used is supplied by AnaSpec (Catolog#72095)


    1. Dilute supplied colorimetric substrate 1:50 in assay buffer

    2. Reconsitute MMP-3/MMP-2 sample with water (500ug/ml)

    3. Mix 2ul MMP-3/MMP-2 and 400ul substrate solution

    4. Take readings @412nm every 10 minutes for ˜90 minutes or until you have reached a steady reading.


    According to the protocol booklet for this AnaSpec kit it states that the first step to activate the pro-MMPs is to indeed incubate with trypsin.

    Read More

    Question

    Hi, This isn't as much of a problem, but I have ordered both MMP 2 and 3 (ab81550 and ab96555) and to test for activity you use a fluorgenic peptide. I want to use these to cleave peptides in solution, and was wondering what conditions you use (what peptide concentration, what is in the buffer, etc)?

    Read More
    Answer

    We use the kit below to test both of these MMP proteins. All assays are run using the instructions in the kit, as well as the buffers they supply.

    http://www.anaspec.com/products/product.asp?id=44834

    Read More

    Question

    Do you have any data suggesting that this will be useful in activity assays of mouse lysates?

    Read More
    Answer

    Thank you for contacting us.

    To our knowledge, this MMP3 protein has not been tested in a mouse system.

    The amino acid sequence of the protein has approximately 78% identity to Mouse MMP3. Though not identical, it may still be capable of cleaving an endogenous mouse substrate, but we have no evidence for this.

    Please do not hesitate to contact us if you need any more advice or information.

    Read More

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