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  1. Link

    products/proteins-peptides/recombinant-human-mmp9-protein-proenzyme-ab82955.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Recombinant Human MMP9 protein (Proenzyme) (ab82955)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
  • SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
  • Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: ELISA, SDS-PAGE, WB

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Description

  • Product name

    Recombinant Human MMP9 protein (Proenzyme)
    See all MMP9 proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    P14780
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      20 to 707
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Corresponding to the pro form of the protein minus the signal peptide with a N-terminal 6X his tag.

Associated products

  • Related Products

    • Anti-MMP9 antibody (ab38898)
    • Anti-MMP9 antibody (ab74277)
    • Anti-MMP9 antibody [EP1254] (ab76003)

Specifications

Our Abpromise guarantee covers the use of ab82955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.20
    Constituents: PBS, 50% Glycerol

General Info

  • Alternative names

    • 82 kDa matrix metalloproteinase-9
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG 4B
    • CLG4B
    • Collagenase Type 4 beta
    • Collagenase type IV 92 KD
    • EC 3.4.24.35
    • Gelatinase 92 KD
    • Gelatinase B
    • Gelatinase beta
    • GelatinaseB
    • GELB
    • Macrophage gelatinase
    • MANDP2
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • Matrix Metalloproteinase 9
    • MMP 9
    • MMP-9
    • MMP9
    • MMP9_HUMAN
    • Type V collagenase
    see all
  • Function

    May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificity

    Produced by normal alveolar macrophages and granulocytes.
  • Involvement in disease

    Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P14780 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
    Sandwich ELISA - Recombinant Human MMP9 protein (Proenzyme) (ab82955)

    Standard curve for MMP9 (Analyte: ab82955); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5µg/ml.

  • SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
    SDS-PAGE - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
    ab82955 on SDS-PAGE.
  • Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955)
    Western blot - Recombinant Human MMP9 protein (Proenzyme) (ab82955)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab82955? Please let us know so that we can cite the reference in this datasheet.

ab82955 has been referenced in 2 publications.

  • Del Ben M  et al. Overexpression of the Vitronectin V10 Subunit in Patients with Nonalcoholic Steatohepatitis: Implications for Noninvasive Diagnosis of NASH. Int J Mol Sci 19:N/A (2018). PubMed: 29463024
  • Matou-Nasri S  et al. Biological impact of advanced glycation endproducts on estrogen receptor-positive MCF-7 breast cancer cells. Biochim Biophys Acta Mol Basis Dis 1863:2808-2820 (2017). PubMed: 28712835

Customer reviews and Q&As

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