Recombinant human NEK9 protein (ab125614)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human NEK9 protein -
Biological activity
The specific activity of ab125614 was determined to be 120 nmol/mg/min.
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Purity
> 75 % SDS-PAGE.
Purity determined to be >75% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
115 kDa including tags -
Amino acids
1 to 979 -
Tags
GST tag N-Terminus -
Additional sequence information
1-979; deleted fragment of 346-731
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.004% EGTA, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- KIAA1995
- APUG
- DKFZp434D0935
see all -
Function
Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. -
Tissue specificity
Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts. -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Contains 6 RCC1 repeats. -
Developmental stage
Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells. -
Domain
Dimerizes through its coiled-coil domain. -
Post-translational
modificationsAutophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
Images
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Functional Studies - Recombinant human NEK9 protein (ab125614)The specific activity of NEK9 (ab125614) was determined to be 121 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human NEK9 protein (ab125614)SDS PAGE analysis of ab125614 -
SDS-PAGE - Recombinant human NEK9 protein (ab125614)SDS PAGE analysis of ab125614 -
Functional Studies - Recombinant human NEK9 protein (ab125614)Sample Kinase Assay showing the specific activity of ab125614 as 120 nmol/min/mg. -
SDS-PAGE - Recombinant human NEK9 protein (ab125614)SDS-PAGE analysis of ab125614.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab125614 has been referenced in 1 publication.
- Lu G et al. NEK9, a novel effector of IL-6/STAT3, regulates metastasis of gastric cancer by targeting ARHGEF2 phosphorylation. Theranostics 11:2460-2474 (2021). PubMed: 33500736