Recombinant human Neuropilin 1 protein (ab204616)
Key features and details
- Expression system: Insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
-
Product name
Recombinant human Neuropilin 1 protein
See all Neuropilin 1 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized soluble Neuropilin 1 binds all VEGF-A isoforms with the exception of VEGF.
-
Purity
> 95 % SDS-PAGE.
Affinity purified. -
Expression system
Insect cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINF NP HFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFL FIKFVS DYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKY PNSLECTYIV FAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWD GFPDVGPHIGRYCG QKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYS VLQSSVSEDFKCMEALGM ESGEIHSDQITASSQYSTNWSAERSRLNYP ENGWTPGEDSYREWIQVDLGLL RFVTAVGTQGAISKETKKKYYVKTYK IDVSSNGEDWITIKEGNKPVLFQGNT NPTDVVVAVFPKPLITRFVRIK PATWETGISMRFEVYGCKITDYPCSGMLGM VSGLISDSQITSSNQGDR NWMPENIRLVTSRSGWALPPAPHSYINEWLQIDL GEEKIVRGIIIQGG KHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFE GNNNYDTPEL RTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPT TPNGNL VDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIKL EH HHHHH -
Predicted molecular weight
83 kDa including tags -
Amino acids
22 to 641 -
Tags
His tag C-Terminus
-
Associated products
-
Related Products
Specifications
Our Abpromise guarantee covers the use of ab204616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute in water or PBS at a concentration of not lower than 100 µg/ml.
General Info
-
Alternative names
- A5 protein
- BDCA4
- BLOOD DENDRITIC CELL ANTIGEN 4
see all -
Function
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. -
Tissue specificity
The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. -
Sequence similarities
Belongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
Images
-
ELISA - Recombinant human Neuropilin 1 protein (ab204616)
Functional ELISA showing binding of VEGF-A isoforms to ab204616 at 1µg/ml (100µl/well), the ligands were added with 10ng/ml.
-
SDS-PAGE - Recombinant human Neuropilin 1 protein (ab204616)SDS PAGE analysis of ab204616 on 10% SDS PAGE gel under reducing conditions stained with Coomassie blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab204616 has not yet been referenced specifically in any publications.