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  1. Link

    products/proteins-peptides/recombinant-human-ornithine-carbamoyltransferaseotc-protein-ab123452.pdf

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Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)

  • Datasheet
  • SDS
Submit a review Q&A (1)

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SDS-PAGE - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)
  • Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 80% SDS-PAGE
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, WB, ELISA

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Protein
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Primary
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Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

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Description

  • Product name

    Recombinant Human Ornithine Carbamoyltransferase/OTC protein
    See all Ornithine Carbamoyltransferase/OTC proteins and peptides
  • Purity

    > 80 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

    P00480
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MLFNLRILLN NAAFRNGHNF MVRNFRCGQP LQNKVQLKGR DLLTLKNFTG EEIKYMLWLS ADLKFRIKQK GEYLPLLQGK SLGMIFEKRS TRTRLSTETG FALLGGHPCF LTTQDIHLGV NESLTDTARV LSSMADAVLA RVYKQSDLDT LAKEASIPII NGLSDLYHPI QILADYLTLQ EHYSSLKGLT LSWIGDGNNI LHSIMMSAAK FGMHLQAATP KGYEPDASVT KLAEQYAKEN GTKLLLTNDP LEAAHGGNVL ITDTWISMGQ EEEKKKRLQA FQGYQVTMKT AKVAASDWTF LHCLPRKPEE VDDEVFYSPR SLVFPEAENR KWTIMAVMVS LLTDYSPQLQKPKFHHHHHH
    • Predicted molecular weight

      41 kDa
    • Amino acids

      1 to 354
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • Form

    Liquid
  • Additional notes

    Product was previously marketed under the MitoSciences sub-brand.

    Previously labelled as Ornithine Carbamoyltransferase. 

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.5
    Constituents: 0.79% Tris HCl, 0.15% EDTA, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

General Info

  • Alternative names

    • EC 2.1.3.3
    • MGC129967
    • MGC129968
    • MGC138856
    • OCTD
    • Ornithine Carbamoyltransferase
    • Ornithine carbamoyltransferase mitochondrial
    • Ornithine carbamoyltransferase, mitochondrial
    • Ornithine transcarbamylase
    • Otc
    • OTC_HUMAN
    • OTCase
    see all
  • Tissue specificity

    Mainly expressed in liver and intestinal mucosa.
  • Pathway

    Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
  • Involvement in disease

    Ornithine carbamoyltransferase deficiency
  • Sequence similarities

    Belongs to the ATCase/OTCase family.
  • Post-translational
    modifications

    Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P00480 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)
    SDS-PAGE - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)
    10-20% acrylamide separation gel showing ab123452 at approximately 40kDa (1µg).
  • Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)
    Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)
    Anti-Ornithine Carbamoyltransferase/OTC antibody (ab86665) at 0.5 µg/ml + Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452) at 0.01 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 5 minutes

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab123452? Please let us know so that we can cite the reference in this datasheet.

ab123452 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Could you provide the sequences for these proteins? We need these to ensure our experiment will work.

Read More

Abcam community

Verified customer

Asked on Dec 24 2012

Answer

Ab58953:
Amino acids 26-530 of UniProt Q5DT02
GKLLV VPMDGSHWFT MQSVVEKLIL RGHEVVVVMP EVSWQLERSL NCTVKTYSTS YTLEDQNREF MVFAHAQWKA QAQSIFSLLM SSSSGFLDLF FSHCRSLFND RKLVEYLKES SFDAVFLDPF DTCGLIVAKY FSLPSVVFTR GIFCHHLEEG AQCPAPLSYV PNDLLGFSDA MTFKERVWNH IVHLEDHLFC QYLFRNALEI ASEILQTPVT AYDLYSHTSI WLLRTDFVLD YPKPVMPNMI FIGGINCHQG KPLPMEFEAY INASGEHGIV VFSLGSMVSE IPEKKAMAIA DALGKIPQTV LWRYTGTRPS NLANNTILVK WLPQNDLLGH PMTRAFITHA GSHGVYESIC NGVPMVMMPL FGDQMDNAKR METKGAGVTL NVLEMTSEDL ENALKAVIND KSYKENIMRL SSLHKDRPVE PLDLAVFWVE FVMRHKGAPH LRPAAHDLTW YQYHSLDVIG FLLAVVLTVA FITFKCCAYG YRKCLGKKGR VKKAHKSKTH


Ab106307:
Amino acids 27-215 of UniProt P15692
APMA EGGGQNHHEV VKFMDVYQRS YCHPIETLVD IFQEYPDEIE YIFKPSCVPL MRCGGCCNDE GLECVPTEES NITMQIMRIK PHQGQHIGEM SFLQHNKCEC RPKKDRARQE KKSVRGKGKG QKRKRKKSRY KSWSVYVGAR CCLMPWSLPG PHPCGPCSER RKHLFVQDPQ TCKCSCKNTD SRCKA


Ab123452:
Amino acids 1-354 of UniProt P00480
MLFNLRILLN NAAFRNGHNF MVRNFRCGQP LQNKVQLKGR DLLTLKNFTG EEIKYMLWLS ADLKFRIKQK GEYLPLLQGK SLGMIFEKRS TRTRLSTETG FALLGGHPCF LTTQDIHLGV NESLTDTARV LSSMADAVLA RVYKQSDLDT LAKEASIPII NGLSDLYHPI QILADYLTLQ EHYSSLKGLT LSWIGDGNNI LHSIMMSAAK FGMHLQAATP KGYEPDASVT KLAEQYAKEN GTKLLLTNDP LEAAHGGNVL ITDTWISMGQ EEEKKKRLQA FQGYQVTMKT AKVAASDWTF LHCLPRKPEE VDDEVFYSPR SLVFPEAENR KWTIMAVMVS LLTDYSPQLQ KPKF

Read More

Abcam Scientific Support

Answered on Dec 24 2012

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