Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
Description
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Product name
Recombinant Human Ornithine Carbamoyltransferase/OTC protein
See all Ornithine Carbamoyltransferase/OTC proteins and peptides -
Purity
> 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MLFNLRILLN NAAFRNGHNF MVRNFRCGQP LQNKVQLKGR DLLTLKNFTG EEIKYMLWLS ADLKFRIKQK GEYLPLLQGK SLGMIFEKRS TRTRLSTETG FALLGGHPCF LTTQDIHLGV NESLTDTARV LSSMADAVLA RVYKQSDLDT LAKEASIPII NGLSDLYHPI QILADYLTLQ EHYSSLKGLT LSWIGDGNNI LHSIMMSAAK FGMHLQAATP KGYEPDASVT KLAEQYAKEN GTKLLLTNDP LEAAHGGNVL ITDTWISMGQ EEEKKKRLQA FQGYQVTMKT AKVAASDWTF LHCLPRKPEE VDDEVFYSPR SLVFPEAENR KWTIMAVMVS LLTDYSPQLQKPKFHHHHHH -
Predicted molecular weight
41 kDa -
Amino acids
1 to 354 -
Tags
His tag C-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab123452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
ELISA
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Form
Liquid -
Additional notes
Product was previously marketed under the MitoSciences sub-brand.
Previously labelled as Ornithine Carbamoyltransferase.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.5
Constituents: 0.79% Tris HCl, 0.15% EDTA, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
General Info
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Alternative names
- EC 2.1.3.3
- MGC129967
- MGC129968
see all -
Tissue specificity
Mainly expressed in liver and intestinal mucosa. -
Pathway
Nitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1. -
Involvement in disease
Ornithine carbamoyltransferase deficiency -
Sequence similarities
Belongs to the ATCase/OTCase family. -
Post-translational
modificationsAcetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)10-20% acrylamide separation gel showing ab123452 at approximately 40kDa (1µg).
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Western blot - Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)Anti-Ornithine Carbamoyltransferase/OTC antibody (ab86665) at 0.5 µg/ml +Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452) at 0.01 µg
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 5 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab123452 has not yet been referenced specifically in any publications.