Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
Product nameRecombinant Human Ornithine Carbamoyltransferase/OTC protein
See all Ornithine Carbamoyltransferase/OTC proteins and peptides
Purity> 80 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMLFNLRILLN NAAFRNGHNF MVRNFRCGQP LQNKVQLKGR DLLTLKNFTG EEIKYMLWLS ADLKFRIKQK GEYLPLLQGK SLGMIFEKRS TRTRLSTETG FALLGGHPCF LTTQDIHLGV NESLTDTARV LSSMADAVLA RVYKQSDLDT LAKEASIPII NGLSDLYHPI QILADYLTLQ EHYSSLKGLT LSWIGDGNNI LHSIMMSAAK FGMHLQAATP KGYEPDASVT KLAEQYAKEN GTKLLLTNDP LEAAHGGNVL ITDTWISMGQ EEEKKKRLQA FQGYQVTMKT AKVAASDWTF LHCLPRKPEE VDDEVFYSPR SLVFPEAENR KWTIMAVMVS LLTDYSPQLQKPKFHHHHHH
Predicted molecular weight41 kDa
Amino acids1 to 354
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab123452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Product was previously marketed under the MitoSciences sub-brand.
Previously labelled as Ornithine Carbamoyltransferase.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.79% Tris HCl, 0.15% EDTA, 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
- EC 18.104.22.168
Tissue specificityMainly expressed in liver and intestinal mucosa.
PathwayNitrogen metabolism; urea cycle; L-citrulline from L-ornithine and carbamoyl phosphate: step 1/1.
Involvement in diseaseOrnithine carbamoyltransferase deficiency
Sequence similaritiesBelongs to the ATCase/OTCase family.
modificationsAcetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
Cellular localizationMitochondrion matrix.
- Information by UniProt
10-20% acrylamide separation gel showing ab123452 at approximately 40kDa (1µg).
Anti-Ornithine Carbamoyltransferase/OTC antibody (ab86665) at 0.5 µg/ml +
Recombinant Human Ornithine Carbamoyltransferase/OTC protein (ab123452) at 0.01 µg
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 5 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
ab123452 has not yet been referenced specifically in any publications.