Recombinant Human Osteopontin protein (Active) (ab281819)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, MS, HPLC, Functional Studies, Cell Culture
Description
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Product name
Recombinant Human Osteopontin protein (Active)
See all Osteopontin proteins and peptides -
Biological activity
Addition of Calcein-labelled HEK-293 cells to immobilized human Osteopontin (≥1 μg) led to 16.6% specific cell adherence.
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Purity
>= 95 % SDS-PAGE.
Equal or greater than 95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN -
Predicted molecular weight
34 kDa -
Actual molecular weight
34 kDa -
Molecular weight information
Mass determination by ESI-TOF. Predicted MW is 33770.58 Da. (+/- 10 Da by ESI-TOF). Observed MW is 33772.69 Da. Additional masses at 33975.94, 34179.02, 34382.33, 34584.90 and 34788.41 are due to residual O-glycans. -
Amino acids
17 to 314 -
Additional sequence information
N-terminal glycine Full-length mature chain lacking the signal peptide.
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Description
Recombinant human Osteopontin protein (Active)
Specifications
Our Abpromise guarantee covers the use of ab281819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
HPLC
Functional Studies
Cell Culture
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
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Alternative names
- BNSP
- Bone sialoprotein 1
- Bone sialoprotein I
see all -
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human Osteopontin protein (Active) (ab281819)
Addition of Calcein-labelled HEK-293 cells to immobilized human Osteopontin (≥1 μg) led to 16.6% specific cell adherence.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3387985-2
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SDS-PAGE - Recombinant Human Osteopontin protein (ab281819)SDS-PAGE analysis of ab281819.
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Mass Spectrometry - Recombinant Human Osteopontin protein (ab281819)Mass determination by ESI-TOF.
Predicted MW is 33770.58 Da. (+/- 10 Da by ESI-TOF). Observed MW is 33772.69 Da. Additional masses at 33975.94, 34179.02, 34382.33, 34584.90 and 34788.41 are due to residual O-glycans.
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HPLC - Recombinant Human Osteopontin protein (ab281819)HPLC analysis of ab281819.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab281819 has not yet been referenced specifically in any publications.
