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  1. Link

    products/proteins-peptides/recombinant-human-p4hb-protein-ab115719.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Recombinant Human P4HB protein (ab115719)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human P4HB protein (ab115719)
  • Western blot - Recombinant Human P4HB protein (ab115719)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: WB, Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant Human P4HB protein
    See all P4HB proteins and peptides
  • Biological activity

    >200U/mg
  • Purity

    > 95 % SDS-PAGE.
    ab115719 is purified by multi-step chromatography.
  • Expression system

    Escherichia coli
  • Accession

    P07237
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      58 kDa
    • Tags

      His tag N-Terminus

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-P4HB antibody (ab96744)

Specifications

Our Abpromise guarantee covers the use of ab115719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    >200U/mg
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Constituents: 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.61% Tris, 0.03% EDTA, 5% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

General Info

  • Alternative names

    • Cellular thyroid hormone binding protein
    • Cellular thyroid hormone-binding protein
    • Collagen prolyl 4 hydroxylase beta
    • Disulphide Isomerase
    • DSI
    • EC 5.3.4.1
    • Endoplasmic reticulum resident protein 59
    • ER protein 59
    • ERBA2L
    • ERp59
    • GIT
    • Gltathione insulin transhydrogenase
    • Glutathione insulin transhydrogenase
    • P4HB
    • P4Hbeta
    • p55
    • PDI
    • PDIA1
    • PDIA1_HUMAN
    • PDIR
    • PHDB
    • PO4DB
    • PO4HB
    • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1)
    • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit
    • PROHB
    • Prolyl 4 hydroxylase beta polypeptide
    • Prolyl 4 hydroxylase beta subunit
    • Prolyl 4 hydroxylase subunit beta
    • Prolyl 4-hydroxylase subunit beta
    • Protein disulfide isomerase associated 1
    • Protein disulfide isomerase, family A, member 1
    • Protein disulfide isomerase/oxidoreductase
    • Protein disulfide-isomerase
    • Protocollagen hydroxylase
    • Thbp
    • Thyroid hormone binding protein p55
    • Thyroid hormone binding protein p55 cellular
    • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like
    see all
  • Function

    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
  • Sequence similarities

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07237 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human P4HB protein (ab115719)
    SDS-PAGE - Recombinant Human P4HB protein (ab115719)
    SDS-PAGE analysis
    Lane 1: MW marker
    Lane 2: ab115719 at 0.5 μg
    Lane 3: ab115719 at 1 μg
    Lane 4: ab115719 at 2 μg
    Lane 5: ab115719 at 5 μg
  • Western blot - Recombinant Human P4HB protein (ab115719)
    Western blot - Recombinant Human P4HB protein (ab115719)
    Monoclonal mouse P4HB antibody + Recombinant Human P4HB protein (ab115719) at 0.05 µg

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab115719? Please let us know so that we can cite the reference in this datasheet.

ab115719 has not yet been referenced specifically in any publications.

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