Recombinant Human P4HB protein (ab115719)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Human P4HB protein
See all P4HB proteins and peptides -
Biological activity
>200U/mg -
Purity
> 95 % SDS-PAGE.
ab115719 is purified by multi-step chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
58 kDa -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab115719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
>200U/mg -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.61% Tris, 0.03% EDTA, 5% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
General Info
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Alternative names
- Cellular thyroid hormone binding protein
- Cellular thyroid hormone-binding protein
- Collagen prolyl 4 hydroxylase beta
see all -
Function
This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
Images
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SDS-PAGE analysis
Lane 1: MW marker
Lane 2: ab115719 at 0.5 μg
Lane 3: ab115719 at 1 μg
Lane 4: ab115719 at 2 μg
Lane 5: ab115719 at 5 μg -
Monoclonal mouse P4HB antibody +
Recombinant Human P4HB protein (ab115719) at 0.05 µg
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab115719 has not yet been referenced specifically in any publications.