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    products/proteins-peptides/recombinant-human-pd-l1-protein-active-ab167713.pdf

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Bioactive grade

Recombinant human PD-L1 protein (Active) (ab167713)

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  • SDS
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Functional Studies - Recombinant human PD-L1 protein (ab167713)
  • Functional Studies - Recombinant human PD-L1 protein (Active) (ab167713)
  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
  • Functional Studies - Recombinant human PD-L1 protein (Active) (ab167713)
  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
  • SDS-PAGE - Recombinant human PD-L1 protein (Active) (ab167713)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 98% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: HPLC, SDS-PAGE, Functional Studies

Achieve higher consistency and quality standards with a premium grade bioactive protein

Product image
Recombinant human PD-L1 protein (Active) (ab280943)
  • High batch-to-batch consistency
  • Optimal bioactivity
  • Guaranteed identical to human native proteins
  • >95% purity
  • Ultra-low endotoxin levels: <0.005 Eu/µg
  • Carrier and tag free

Description

  • Product name

    Recombinant human PD-L1 protein (Active)
    See all PD-L1 proteins and peptides
  • Biological activity

    Immobilized ab167713 on Anti-His Tag (C-term) Antibody, precoated (0.1 μg/well) plate, binds Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified), at 5 μg/mL (100 μL/well) with a linear range of 10-78 ng/mL.

  • Purity

    > 98 % SDS-PAGE.
    >90% as determined by SEC-HPLC. Purified by Immobilized metal affinity chromatography.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    Q9NZQ7
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFV HGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISY GGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWT SSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEE NHTAELVIPELPLAHPPNER
    • Predicted molecular weight

      26 kDa including tags
    • Molecular weight information

      The protein migrates as 30-35 kDa under reducing conditions due to glycosylation.
    • Amino acids

      19 to 238
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NP_054862.1)

Associated products

  • Corresponding Antibody

    • Anti-PD-L1 antibody [28-8] (ab205921)
  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Recombinant human PD1 protein (Fc Chimera Active) (ab221398)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab167713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

    Functional Studies

  • Form

    Lyophilized
  • Additional notes

    For a recombinant rabbit monoclonal antiody to PD-L1 in IHC usage and KO validated - see ab205921 (clone 28-8)

    For a recombinant rabbit monoclonal antiody to PD-L1 in WB and ICC/IF usage - see ab174838 (clone EPR1161(2))

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    0.22 µm filtered

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 400 µg/ml.

General Info

  • Alternative names

    • B7 H
    • B7 H1
    • B7 homolog 1
    • B7-H1
    • B7H
    • B7H1
    • CD 274
    • CD-274
    • CD274
    • CD274 antigen
    • CD274 molecule
    • MGC142294
    • MGC142296
    • OTTHUMP00000021029
    • PD L1
    • PD-L1
    • PD1L1_HUMAN
    • PDCD1 ligand 1
    • PDCD1L1
    • PDCD1LG1
    • PDL 1
    • PDL1
    • Programmed cell death 1 ligand 1
    • Programmed death ligand 1
    • RGD1566211
    see all
  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane and Endomembrane system.
  • Target information above from: UniProt accession Q9NZQ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
    Functional Studies - Recombinant human PD-L1 protein (ab167713)

    Immobilized ab167713 at 1 μg/mL (100 μL/well) binds Anti-Human PD-L1 MAb (Human IgG1).

    Linear range of 0.1-3 ng/mL (QC tested).

  • Functional Studies - Recombinant human PD-L1 protein (Active) (ab167713)
    Functional Studies - Recombinant human PD-L1 protein (Active) (ab167713)
    The purity of Human PD-L1 (His Tag) (HPLC verified) was greater than 90% as determined by SEC-HPLC.
  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
    Functional Studies - Recombinant human PD-L1 protein (ab167713)

    Immobilized ab167713 on Anti-His Tag (C-term) Antibody, precoated (0.1 μg/well) plate, binds Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified), at 5 μg/mL (100 μL/well).

    Linear range of 10-78 ng/mL (Routinely tested).

  • Functional Studies - Recombinant human PD-L1 protein (Active) (ab167713)
    Functional Studies - Recombinant human PD-L1 protein (Active) (ab167713)

    Loaded Recombinant human PD1 protein (Fc Chimera Active) ab221398 on ProteinA Biosensor, can bind Human PD-L1, His Tag ab167713 with an affinity constant of 5.3 μM as determined in BLI assay.

  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
    Functional Studies - Recombinant human PD-L1 protein (ab167713)

    Biotinylated Human PD-1, Fc Tag (ab246137) binds Recombinant human PD-L1 protein ab167713 at 1 μg/mL (5μL/well).

    Linear range of 0.02-0.625 μg/mL as determined in a alphaLISA homogeneous assay (Routinely tested).

  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
    Functional Studies - Recombinant human PD-L1 protein (ab167713)

    Human PD-1, Fc Tag (ab221398), captured on CM5 chip via anti-human IgG Fc antibody, binds ab167713 with an affinity constant of 3.6 μM, as determined in an SPR assay (Biacore T200).

  • Functional Studies - Recombinant human PD-L1 protein (ab167713)
    Functional Studies - Recombinant human PD-L1 protein (ab167713)

    Anti-Human PD-L1 Mab (Human IgG1), captured on CM5 chip via anti-human IgG Fc antibodies surface, binds ab167713 with an affinity constant of 0.286 nM, as determined in a SPR assay (Biacore T200) (Routinely tested).

  • SDS-PAGE - Recombinant human PD-L1 protein (Active) (ab167713)
    SDS-PAGE - Recombinant human PD-L1 protein (Active) (ab167713)

    Reduced ab167713 on SDS-PAGE, stained overnight with Coomassie Blue.

    The purity of the protein is greater than 98%.

    The protein migrates as 30-35 kDa under reducing conditions, due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab167713? Please let us know so that we can cite the reference in this datasheet.

ab167713 has not yet been referenced specifically in any publications.

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