Recombinant human PD-L1 protein (Active) (ab280943)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: MS, Sandwich ELISA, SDS-PAGE, HPLC, Functional Studies
Description
-
Product name
Recombinant human PD-L1 protein (Active)
See all PD-L1 proteins and peptides -
Biological activity
Fully biologically active measured by its ability to inhibit anti-CD3 and anti-CD8 antibody induced T-cell expansion. ED50 for this effect is ≤500 ng/mL with a specific activity of 0.2 x 104 units/mg.
-
Purity
>= 95 % SDS-PAGE.
=95% by HPLC -
Endotoxin level
<=0.005 Eu/µg -
Expression system
HEK 293 cells -
Accession
-
Protein length
Protein fragment -
Animal free
Yes -
Carrier free
Yes -
Nature
Recombinant -
-
Species
Human -
Sequence
FTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER -
Predicted molecular weight
25 kDa -
Actual molecular weight
25 kDa -
Molecular weight information
Predicted MW is 25250.68 Da (+/- 10 Da by ESI-TOF). Observed MW is 25097.90 Da. M-100 due to loss of C-terminal arginine. -
Amino acids
19 to 238 -
Additional sequence information
N-terminal glycine.
-
Specifications
Our Abpromise guarantee covers the use of ab280943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Mass Spectrometry
Sandwich ELISA
SDS-PAGE
HPLC
Functional Studies
-
Form
Lyophilized -
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionReconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
General Info
-
Alternative names
- B7 H
- B7 H1
- B7 homolog 1
see all -
Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
Images
-
Functional Studies - Recombinant human PD-L1 protein (ab280943)
Functional analysis of an280943.
Fully biologically active measured by its ability to inhibit anti-CD3 and anti-CD8 antibody induced T-cell expansion. ED50 for this effect is ≤500 ng/mL with a specific activity of 0.2 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3381126-2
-
SDS-PAGE - Recombinant Human PD-L1 protein (ab280943)SDS-PAGE analysis of ab280943.
-
Sandwich ELISA - Recombinant human PD-L1 protein (Active) (ab280943)Sandwich ELISA - Recombinant human PD-L1 protein standard curve.
Background subtracted standard curve using Human/Monkey PD-L1 [28-8] Antibody Pair - BSA and Azide free (ab253485) and Recombinant human PD-L1 protein (Active) (ab280943) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
-
Mass Spectrometry - Recombinant Human PD-L1 protein (ab280943)Mass determination by ESI-TOF.
Predicted MW is 25250.68 Da (+/- 10 Da by ESI-TOF). Observed MW is 25097.90 Da. M-100 due to loss of C-terminal arginine.
-
HPLC - Recombinant Human PD-L1 protein (ab280943)HPLC analysis of ab280943.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab280943 has not yet been referenced specifically in any publications.
