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    products/proteins-peptides/recombinant-human-pd1-protein-active-ab174035.pdf

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Bioactive grade

Recombinant human PD1 protein (Active) (ab174035)

  • Datasheet
Submit a review Submit a question References (1)

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ELISA - Recombinant human PD1 protein (Active) (ab174035)
  • Functional Studies - Recombinant human PD1 protein (ab174035)
  • Functional Studies - Recombinant human PD1 protein (Active) (ab174035)
  • Functional Studies - Recombinant human PD1 protein (ab174035)
  • Functional Studies - Recombinant human PD1 protein (Active) (ab174035)
  • Functional Studies - Recombinant human PD1 protein (ab174035)
  • Functional Studies - Recombinant human PD1 protein (ab174035)
  • Functional Studies - Recombinant human PD1 protein (ab174035)
  • SDS-PAGE - Recombinant human PD1 protein (ab174035)
  • HPLC - Recombinant human PD1 protein (ab174035)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: HPLC, SDS-PAGE, Functional Studies, ELISA

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Description

  • Product name

    Recombinant human PD1 protein (Active)
    See all PD1 proteins and peptides
  • Biological activity

    Measured by its binding ability in a functional ELISA.

    Immobilized PD1 at 10 μg/ml (100 μl/well) can bind recombinant Human B7-H1 Fc chimera with a linear range of 0.005- 0.4 μg/ml.

  • Purity

    > 95 % SDS-PAGE.
    >90% as determined by SEC-HPLC. Purified by Immobilized metal affinity chromatography.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    HEK 293 cells
  • Accession

    Q15116
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSN QTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCG AISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ
    • Predicted molecular weight

      17 kDa including tags
    • Amino acids

      25 to 167
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NP_005009.2)

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)
    • Anti-6X His tag® antibody (ab9108)

Specifications

Our Abpromise guarantee covers the use of ab174035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    SDS-PAGE

    Functional Studies

    ELISA

  • Form

    Lyophilized
  • Additional notes

    For long term storage, store at lyophlized state at -20°C or lower. After reconstitution, store under sterile conditions for 3 months at -70°C or 12 months at 4-8°C at lyophlized state. Avoid repeated freeze-thaw cycles.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    The formulation is lot specific. Please contact our Technical Support team for details

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    This information is lot specific. Please contact our Technical Support team for details

General Info

  • Alternative names

    • CD279
    • CD279 antigen
    • hPD 1
    • hPD l
    • hPD-1
    • hSLE1
    • PD 1
    • PD-1
    • PD1
    • PDCD 1
    • PDCD1
    • PDCD1_HUMAN
    • Programmed cell death 1
    • Programmed cell death 1 protein
    • Programmed cell death protein 1
    • Protein PD 1
    • Protein PD-1
    • SLEB2
    • Systemic lupus erythematosus susceptibility 2
    see all
  • Function

    Possible cell death inducer, in association with other factors.
  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage

    Induced at programmed cell death.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q15116 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • ELISA - Recombinant human PD1 protein (Active) (ab174035)
    ELISA - Recombinant human PD1 protein (Active) (ab174035)

    Immobilized ab174035 at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL.

  • Functional Studies - Recombinant human PD1 protein (ab174035)
    Functional Studies - Recombinant human PD1 protein (ab174035)

    Immobilized ab174035 at 0.2μg/mL (100 μL/well) binds Human PD-L1, Fc Tag.

    Linear range of 0.31-1.25 μg/mL (QC tested).

  • Functional Studies - Recombinant human PD1 protein (Active) (ab174035)
    Functional Studies - Recombinant human PD1 protein (Active) (ab174035)
    Loaded Human PD-1 (His Tag) (HPLC verified) on HIS1K Biosensor can bind Human PD-L2 (Fc Tag) (HPLC verified) with an affinity constant of 16.3 nM as determined in bli assay (ForteBio Octet Red96e) (QC tested).
  • Functional Studies - Recombinant human PD1 protein (ab174035)
    Functional Studies - Recombinant human PD1 protein (ab174035)

    Immobilized ab174035 at 0.2 μg/mL ( 100 μl/well ) binds Human PD-L2, Fc Tag. 

    Linear range of 0.16-2.5 μg/mL (QC tested).

  • Functional Studies - Recombinant human PD1 protein (Active) (ab174035)
    Functional Studies - Recombinant human PD1 protein (Active) (ab174035)

    Loaded Human PD-1 (His Tag) (HPLC verified) on HIS1K Biosensor can bind Human PD-L1 (Fc Tag) (HPLC verified) with an affinity constant of 38.9 nM as determined in bli assay (ForteBio Octet Red96e) (QC tested).

  • Functional Studies - Recombinant human PD1 protein (ab174035)
    Functional Studies - Recombinant human PD1 protein (ab174035)

    Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Human PD-L2, Mouse IgG1 Fc Tag.

    Linear range of 10-156 ng/mL (Routinely tested).

  • Functional Studies - Recombinant human PD1 protein (ab174035)
    Functional Studies - Recombinant human PD1 protein (ab174035)

    Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Nivolumab.

    Linear range of 0.1-3 ng/mL (Routinely tested).

  • Functional Studies - Recombinant human PD1 protein (ab174035)
    Functional Studies - Recombinant human PD1 protein (ab174035)

    Opdivo(Nivolumab), captured on CM5 chip via anti-human IgG Fc antibodies surface, binds ab174035 with an affinity constant of 4.94 nM, as determined in an SPR assay (Biacore T200) (Routinely tested).

  • SDS-PAGE - Recombinant human PD1 protein (ab174035)
    SDS-PAGE - Recombinant human PD1 protein (ab174035)

    Reduced ab174035 on SDS-PAGE, stained overnight with Coomassie Blue.

    The purity of the protein is greater than 95%.

    The protein migrates as 31-44kDa under reducing conditions, due to glycosylation.

  • HPLC - Recombinant human PD1 protein (ab174035)
    HPLC - Recombinant human PD1 protein (ab174035)

    The purity of ab174035 was greater than 90%, as determined by SEC-HPLC.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab174035? Please let us know so that we can cite the reference in this datasheet.

ab174035 has been referenced in 1 publication.

  • Fang J  et al. aPD-1-mesoCAR-T cells partially inhibit the growth of advanced/refractory ovarian cancer in a patient along with daily apatinib. J Immunother Cancer 9:N/A (2021). PubMed: 33589520

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