Recombinant human PD1 protein (Active) (ab174035)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: HPLC, SDS-PAGE, Functional Studies, ELISA
Description
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Product name
Recombinant human PD1 protein (Active)
See all PD1 proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA.
Immobilized PD1 at 10 μg/ml (100 μl/well) can bind recombinant Human B7-H1 Fc chimera with a linear range of 0.005- 0.4 μg/ml.
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Purity
> 95 % SDS-PAGE.
>90% as determined by SEC-HPLC. Purified by Immobilized metal affinity chromatography. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSN QTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCG AISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ -
Predicted molecular weight
17 kDa including tags -
Amino acids
25 to 167 -
Tags
His tag C-Terminus -
Additional sequence information
(NP_005009.2)
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174035 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
SDS-PAGE
Functional Studies
ELISA
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Form
Lyophilized -
Additional notes
For long term storage, store at lyophlized state at -20°C or lower. After reconstitution, store under sterile conditions for 3 months at -70°C or 12 months at 4-8°C at lyophlized state. Avoid repeated freeze-thaw cycles.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.
pH: 7.40
Constituents: PBS, 5% Trehalose
The formulation is lot specific. Please contact our Technical Support team for detailsThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionThis information is lot specific. Please contact our Technical Support team for details
General Info
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
Images
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ELISA - Recombinant human PD1 protein (Active) (ab174035)
Immobilized ab174035 at 2 μg/mL (100 μL/well) can bind Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL.
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Functional Studies - Recombinant human PD1 protein (ab174035)Immobilized ab174035 at 0.2μg/mL (100 μL/well) binds Human PD-L1, Fc Tag.
Linear range of 0.31-1.25 μg/mL (QC tested).
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Functional Studies - Recombinant human PD1 protein (Active) (ab174035)Loaded Human PD-1 (His Tag) (HPLC verified) on HIS1K Biosensor can bind Human PD-L2 (Fc Tag) (HPLC verified) with an affinity constant of 16.3 nM as determined in bli assay (ForteBio Octet Red96e) (QC tested). -
Functional Studies - Recombinant human PD1 protein (ab174035)Immobilized ab174035 at 0.2 μg/mL ( 100 μl/well ) binds Human PD-L2, Fc Tag.
Linear range of 0.16-2.5 μg/mL (QC tested).
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Functional Studies - Recombinant human PD1 protein (Active) (ab174035)Loaded Human PD-1 (His Tag) (HPLC verified) on HIS1K Biosensor can bind Human PD-L1 (Fc Tag) (HPLC verified) with an affinity constant of 38.9 nM as determined in bli assay (ForteBio Octet Red96e) (QC tested).
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Functional Studies - Recombinant human PD1 protein (ab174035)Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Human PD-L2, Mouse IgG1 Fc Tag.
Linear range of 10-156 ng/mL (Routinely tested).
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Functional Studies - Recombinant human PD1 protein (ab174035)Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Nivolumab.
Linear range of 0.1-3 ng/mL (Routinely tested).
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Functional Studies - Recombinant human PD1 protein (ab174035)Opdivo(Nivolumab), captured on CM5 chip via anti-human IgG Fc antibodies surface, binds ab174035 with an affinity constant of 4.94 nM, as determined in an SPR assay (Biacore T200) (Routinely tested).
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SDS-PAGE - Recombinant human PD1 protein (ab174035)Reduced ab174035 on SDS-PAGE, stained overnight with Coomassie Blue.
The purity of the protein is greater than 95%.
The protein migrates as 31-44kDa under reducing conditions, due to glycosylation.
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HPLC - Recombinant human PD1 protein (ab174035)The purity of ab174035 was greater than 90%, as determined by SEC-HPLC.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab174035 has been referenced in 2 publications.
- Zhou L et al. Homodimerized cytoplasmic domain of PD-L1 regulates its complex glycosylation in living cells. Commun Biol 5:887 (2022). PubMed: 36042378
- Fang J et al. aPD-1-mesoCAR-T cells partially inhibit the growth of advanced/refractory ovarian cancer in a patient along with daily apatinib. J Immunother Cancer 9:N/A (2021). PubMed: 33589520
