Recombinant human PD1 protein (Tagged) (Biotin) (ab271670)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, DDDDK tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human PD1 protein (Tagged) (Biotin)
See all PD1 proteins and peptides -
Biological activity
Assay Conditions: Briefly, 50 µl of 2 µg/ml PD-L1 was coated onto plates. 20 µl of 0.5 µg/ml PD-1, FLAG-His, Biotin-labelled was allowed to bind at room temperature for 2 hours. Strep-HRP was added and allowed to incubate at room temp for 1 hr, followed by HPR chemiluminescent substrate addition and plate reading.
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Purity
>= 80 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LDSPDRPWNP PTFSPALLVV TEGDNATFTC SFSNTSESFV LNWYRMSPSN QTDKLAAFPE DRSQPGQDCR FRVTQLPNGR DFHMSVVRAR RNDSGTYLCG AISLAPKAQI KESLRAELRV TERRAEVPTA HPSPSPRPAG QFQ -
Predicted molecular weight
20 kDa including tags -
Molecular weight information
This protein runs at a higher molecular weight by SDS-PAGE due to glycosylation. -
Amino acids
25 to 167 -
Modifications
biotinylated -
Tags
His tag C-Terminus , DDDDK tag C-Terminus , Avi tag C-Terminus
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Conjugation
Biotin
Specifications
Our Abpromise guarantee covers the use of ab271670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
Enzymatically biotin-labeled using Avi-tag™ technology
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PD1 protein (Tagged) (Biotin) (ab271670)SDS-PAGE analysis of ab271670.
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Functional Studies - Recombinant human PD1 protein (Tagged) (Biotin) (ab271670)Binding activity of ab271670.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab271670 has not yet been referenced specifically in any publications.