Recombinant human PDGFR alpha protein (ab84797)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies
Description
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Product name
Recombinant human PDGFR alpha protein
See all PDGFR alpha proteins and peptides -
Biological activity
Specific activity: 12nmol/min/mg.
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Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
95 kDa -
Amino acids
550 to 1089 -
Tags
GST tag N-Terminus -
Additional sequence information
Recombinant fragment of Human PDGF Receptor alpha (550-end) (cytoplasmic domain).
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab84797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Alpha-type platelet-derived growth factor receptor
- CD140 antigen-like family member A
- CD140a
see all -
Function
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human PDGFR alpha protein (ab84797)The specific activity of PDGFR alpha (ab84797) was determined to be 12.5 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PDGFR alpha protein (ab84797)SDS PAGE analysis of ab84797 -
SDS-PAGE - Recombinant human PDGFR alpha protein (ab84797)SDS PAGE analysis of ab84797 -
Functional Studies - Recombinant human PDGFR alpha protein (ab84797)Activity of ab84797 -
SDS-PAGE - Recombinant human PDGFR alpha protein (ab84797)SDS Page analysis of ab84797
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab84797 has not yet been referenced specifically in any publications.