Recombinant Human Periostin protein (ab203522)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB, ELISA
Description
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Product name
Recombinant Human Periostin protein
See all Periostin proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGHHHHHHHHHHSSGHIEGRHMRNNHYDKILAHSRIRGRDQGPNVCALQQ ILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPI DHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSD IRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINH YPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLS SFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERFMGDKVASE ALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNK KDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALR PDGEYTLLAPVNNAFSDDTLSMVQRLLKLILQNHILKVKVGLNELYNGQI LETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKS LHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEE KEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEV NDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLI KYIQIKFVRGSTFKEIPVTVY -
Predicted molecular weight
75 kDa including tags -
Amino acids
22 to 669 -
Tags
His tag N-Terminus -
Additional sequence information
Also contains an Xa – cleavage site at amino acid 23.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab203522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
ELISA
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Form
Lyophilized -
Additional notes
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
0.05 M Acetate buffer
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ReconstitutionAdd 0.1 M acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
General Info
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Alternative names
- Fasciclin-I like
- MGC119510
- MGC119511
see all -
Function
Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. -
Tissue specificity
Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. -
Sequence similarities
Contains 1 EMI domain.
Contains 4 FAS1 domains. -
Post-translational
modificationsGamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Periostin protein (ab203522)
12% SDS-PAGE analysis of ab203522.
Lane 1: Reduced and heated sample, 5 μg/lane
Lane 2: Non-reduced and non-heated sample, 5 μg/lane
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab203522 has not yet been referenced specifically in any publications.
