Recombinant human PKC alpha protein (Active) (ab55672)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 95% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, WB, SDS-PAGE
Description
-
Product name
Recombinant human PKC alpha protein (Active)
See all PKC alpha proteins and peptides -
Biological activity
Specific Activity: 3,200 nmol/min/mg.
-
Purity
> 95 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MADVFPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSVCK GLMTKHPAKR LGCGPEGERD VREHAFFRRI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV -
Predicted molecular weight
103 kDa -
Amino acids
1 to 672 -
Tags
GST tag N-Terminus -
Additional sequence information
(NM_002737)
-
Associated products
-
Related Products
-
Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab55672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
Functional Studies
Western blot
SDS-PAGE
-
Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Avoid repeated handling.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride, 0.31% GlutathioneThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- AAG6
- Aging associated gene 6
- aPKC
see all -
Function
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. - Information by UniProt
Images
-
Functional Studies - Recombinant human PKC alpha protein (ab55672)
Specific activity: 3,200 nmol/min/mg
-
SDS-PAGE - Recombinant human PKC alpha protein (ab55672)Purity >95% by densiometry.
-
Western blot - Recombinant human PKC alpha protein (ab55672)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab55672 has been referenced in 4 publications.
- Huang F et al. Reversible phosphorylation of cyclin T1 promotes assembly and stability of P-TEFb. Elife 10:N/A (2021). PubMed: 34821217
- Ahmad S et al. Electrochemical detection of neuronal extracellular phosphorylation by PKA, PKC and Src. Anal Biochem 608:113892 (2020). PubMed: 32810472
- Ahmad S et al. Electrochemical studies of human nAChR a7 subunit phosphorylation by kinases PKA, PKC and Src. Anal Biochem 574:46-56 (2019). PubMed: 30914244
- Lear TB et al. KIAA0317 regulates pulmonary inflammation through SOCS2 degradation. JCI Insight 4:N/A (2019). PubMed: 31578312