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    products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC
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Recombinant human PKC beta 2 protein (ab60841)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human PKC beta 2 protein (ab60841)
  • SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
  • SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
  • Functional Studies - Recombinant human PKC beta 2 protein (ab60841)
  • SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 70% Affinity purified
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

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Description

  • Product name

    Recombinant human PKC beta 2 protein
  • Biological activity

    423 nmol/min/mg Specific activity defined by radiometric assay, nmol of gamma-phosphate from ATP transferred to PKCtide peptide substrate

  • Purity

    > 70 % Affinity purified.
    Purified by affinity chromatography
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    P05771-2
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MADPAAGPPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS KHKFKIHTYS SPTFCDHCGS LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL CGTDHTERRG RIYIQAHIDR DVLIVLVRDA KNLVPMDPNG LSDPYVKLKL IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL SVEIWDWDLT SRNDFMGSLS FGISELQKAS VDGWFKLLSQ EEGEYFNVPV PPEGSEANEE LRQKFERAKI SQGTKVPEEK TTNTVSKFDN NGNRDRMKLT DFNFLMVLGK GSFGKVMLSE RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKEHAFF RYIDWEKLER KEIQPPYKPK ACGRNAENFD RFFTRHPPVL TPPDQEVIRN IDQSEFEGFS FVNSEFLKPE VKS
    • Predicted molecular weight

      105 kDa
    • Amino acids

      1 to 671
    • Tags

      GST tag N-Terminus

Associated products

  • Related Products

    • Anti-PKC beta 2 antibody [Y125] (ab32026)
  • Substrate reagent

    • CREB peptide (ab204856)

Specifications

Our Abpromise guarantee covers the use of ab60841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.31% Glutathione

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • KPCB_HUMAN
    • PKC Beta
    • PKC-B
    • PKC-beta
    • PKCB
    • Prkcb
    • PRKCB II
    • PRKCB2
    • Protein kinase C beta
    • Protein kinase C beta type
    see all
  • Function

    Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localization

    Cytoplasm. Nucleus. Membrane.
  • Target information above from: UniProt accession P05771 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human PKC beta 2 protein (ab60841)
    Functional Studies - Recombinant human PKC beta 2 protein (ab60841)
    The specific activity of PKC beta 2 (ab60841) was determined to be 423 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
    SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
    SDS PAGE analysis of ab60841
  • SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
    SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
    SDS PAGE analysis of ab60841
  • Functional Studies - Recombinant human PKC beta 2 protein (ab60841)
    Functional Studies - Recombinant human PKC beta 2 protein (ab60841)
    Sample Kinase Activity Plot.
  • SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
    SDS-PAGE - Recombinant human PKC beta 2 protein (ab60841)
    ab60841 on SDS-PAGE, MW ~105 kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab60841? Please let us know so that we can cite the reference in this datasheet.

ab60841 has been referenced in 2 publications.

  • Ferreira JCB  et al. A selective inhibitor of mitofusin 1-ßIIPKC association improves heart failure outcome in rats. Nat Commun 10:329 (2019). PubMed: 30659190
  • Kori S  et al. Structure of the UHRF1 Tandem Tudor Domain Bound to a Methylated Non-histone Protein, LIG1, Reveals Rules for Binding and Regulation. Structure 27:485-496.e7 (2019). PubMed: 30639225

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