Recombinant human PKN2 protein (ab89860)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
Description
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Product name
Recombinant human PKN2 protein -
Biological activity
The specific activity of ab89860 was determined to be 74 nmol/min/mg
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Purity
> 75 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab89860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
Functional Studies
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cardiolipin activated protein kinase Pak2
- MGC150606
- MGC71074
see all -
Function
Exhibits a preference for highly basic protein substrates. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 1 protein kinase domain.
Contains 3 REM (Hr1) repeats. -
Domain
The C1 domain does not bind the diacylglycerol (DAG). -
Post-translational
modificationsAutophosphorylated.
Activated by limited proteolysis with trypsin. -
Cellular localization
Cytoplasm. - Information by UniProt
Images
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Functional Studies - Recombinant human PKN2 protein (ab89860)The specific activity of PKN2 (ab89860) was determined to be 78 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PKN2 protein (ab89860)SDS PAGE analysis of ab89860 -
SDS-PAGE - Recombinant human PKN2 protein (ab89860)SDS PAGE analysis of ab89860 -
Functional Studies - Recombinant human PKN2 protein (ab89860)Kinase Assay demonstrating specific activity of ab89860 -
SDS-PAGE - Recombinant human PKN2 protein (ab89860)SDS-PAGE showing ab89860 at approximately 145kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab89860 has not yet been referenced specifically in any publications.