Recombinant human PRKACG protein (ab60838)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 60% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PRKACG protein -
Purity
> 60 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Tags
GST tag N-Terminus
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204856 (CREB peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as PKA gamma (catalytic subunit)
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- cAMP-dependent protein kinase catalytic subunit gamma
- KAPCG_HUMAN
- KAPG
see all -
Function
Phosphorylates a large number of substrates in the cytoplasm and the nucleus. -
Tissue specificity
Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - Information by UniProt
Images
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Functional Studies - Recombinant human PRKACG protein (ab60838)The specific activity of PRKACG (ab60838) was determined to be 174 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PRKACG protein (ab60838)SDS PAGE analysis of ab60838 -
SDS-PAGE - Recombinant human PRKACG protein (ab60838)SDS PAGE analysis of ab60838 -
Functional Studies - Recombinant human PRKACG protein (ab60838)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human PRKACG protein (ab60838)ab60838 on SDS-PAGE, MW ~65 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab60838 has not yet been referenced specifically in any publications.