Recombinant human Prorenin protein (Active) (ab93266)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
Product nameRecombinant human Prorenin protein (Active)
See all Prorenin proteins and peptides
Fully activatable to renin by catalytic amounts of trypsin.
ab93266 is > 95% active.
Purity> 95 % SDS-PAGE.
ab93266 is purified by affinity chromatography.
Expression systemHEK 293 cells
Protein lengthFull length protein
Predicted molecular weight44 kDa
Our Abpromise guarantee covers the use of ab93266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Extinction coefficient: 1.1
UV Absorbance (280 nm)=0.99
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.79% Tris HCl, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Angiotensin forming enzyme
FunctionRenin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Involvement in diseaseDefects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
Sequence similaritiesBelongs to the peptidase A1 family.
Cellular localizationSecreted. Membrane. Associated to membranes via binding to ATP6AP2.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
ab93266 has been referenced in 3 publications.
- Ooi K et al. Sigma-1 Receptor Activation Suppresses Microglia M1 Polarization via Regulating Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Functions in Stress-Induced Hypertension Rats. Mol Neurobiol 58:6625-6646 (2021). PubMed: 34601668
- Hu L et al. Microglia-Derived NLRP3 Activation Mediates the Pressor Effect of Prorenin in the Rostral Ventrolateral Medulla of Stress-Induced Hypertensive Rats. Neurosci Bull 36:475-492 (2020). PubMed: 32242284
- Hu L et al. Melatonin decreases M1 polarization via attenuating mitochondrial oxidative damage depending on UCP2 pathway in prorenin-treated microglia. PLoS One 14:e0212138 (2019). PubMed: 30742657