Recombinant Human PRP19 protein (BSA and azide free) (ab172187)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Tags: proprietary tag N-Terminus
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human PRP19 protein (BSA and azide free) -
Purity
> 90 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Carrier free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
SLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQ LIDIKVAHPIRPKPPSATSIPAILKALQDEWDAVMLHSFTLRQQLQTTRQ ELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGLIVPQAVPSSQP SVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKP EELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDK SSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQV VRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGC SLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFS ENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLA LGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKF YSL -
Predicted molecular weight
55 kDa -
Amino acids
2 to 504 -
Tags
proprietary tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab172187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.29% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- hPso4
- NMP200
- Nuclear matrix protein 200
see all -
Function
Plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells. -
Tissue specificity
Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines. -
Sequence similarities
Belongs to the WD repeat PRP19 family.
Contains 1 U-box domain.
Contains 7 WD repeats. -
Cellular localization
Nucleus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Nucleoplasmic in interphase cells. Irregularly distributed in anaphase cells. In prophase cells, uniformly distributed, but not associated with condensing chromosomes. Found in extrachromosomal regions in metaphase cells. Mainly localized to the mitotic spindle apparatus when chromosomes segregate during anaphase. When nuclei reform during late telophase, uniformly distributed in daughter cells and displays no preferred association with decondensing chromatin. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PRP19 protein (BSA and azide free) (ab172187)
SDS-PAGE showing ab172187. Approx. MW ~74kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab172187 has not yet been referenced specifically in any publications.