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    products/proteins-peptides/recombinant-human-rhoc-protein-ab98085.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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Recombinant Human RHOC protein (ab98085)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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SDS-PAGE - Recombinant Human RHOC protein (ab98085)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, MS

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    Description

    • Product name

      Recombinant Human RHOC protein
      See all RHOC proteins and peptides
    • Purity

      > 90 % SDS-PAGE.
      ab98085 was purified using conventional chromatography techniques.
    • Expression system

      Escherichia coli
    • Accession

      P08134
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHHSSGLVPRGSHMAAIRKKLVIVGDGACGKTCLLIVFSKDQF PEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVI LMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRE LAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQ VRKNKRRRGC
      • Predicted molecular weight

        24 kDa including tags
      • Amino acids

        1 to 190
      • Tags

        His tag N-Terminus

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)

    Specifications

    Our Abpromise guarantee covers the use of ab98085 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Mass Spectrometry

    • Mass spectrometry

      MALDI-TOF
    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0154% DTT, 0.316% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • Aplysia RAS-related homolog 9
      • ARH 9
      • ARH9
      • ARHC
      • H9
      • MGC1448
      • MGC61427
      • Oncogene RHO H9
      • Ras homolog gene family member C
      • RAS related homolog 9
      • RHO C
      • Rho cDNA clone 9
      • Rho related GTP binding protein RhoC
      • Rho-related GTP-binding protein RhoC
      • rhoC
      • RhoC GTPase
      • RHOC_HUMAN
      • RHOH 9
      • RHOH9
      • Small GTP binding protein RhoC
      see all
    • Function

      Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Rho family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P08134 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human RHOC protein (ab98085)
      SDS-PAGE - Recombinant Human RHOC protein (ab98085)
      15% SDS-PAGE analysis of 3µg ab98085

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab98085? Please let us know so that we can cite the reference in this datasheet.

    ab98085 has been referenced in 1 publication.

    • Khan A  et al. Tumor necrosis factor-induced ArhGEF10 selectively activates RhoB contributing to human microvascular endothelial cell tight junction disruption. FASEB J 35:e21627 (2021). PubMed: 33948992

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