Recombinant Human Serum Albumin protein (His tag) (ab217817)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant Human Serum Albumin protein (His tag)
See all Human Serum Albumin proteins and peptides -
Purity
> 95 % SDS-PAGE.
>95% as determined by SDS-PAGE. >90% as determined by SEC-MALS. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
HEK 293 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFA KTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNE CFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFY APELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKC ASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDL LECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLA KTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGE YKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAE DYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPK EFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDD FAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL -
Predicted molecular weight
67 kDa including tags -
Amino acids
25 to 609 -
Tags
His tag C-Terminus -
Additional sequence information
The predicted N-terminus is Asp 25. This product is the mature full length protein from aa 25 to 609. The signal peptide and propeptide are not included (NP_000468).
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Description
Recombinant Human Human Serum Albumin protein (His tag)
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab217817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 90% PBS, 10% Trehalose
Lyophilised from 0.22 µm filtered solution. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- alb
- ALBU_HUMAN
- Albumin
see all -
Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Serum Albumin protein (His tag) (ab217817)
SDS-PAGE analysis of DTT-reduced ab217817 followed by staining overnight with Coomassie Blue.
DTT-reduced protein migrates as 66 kDa in SDS-PAGE due to glycosylation.
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Size Exclusion Chromatography - Recombinant Human Serum Albumin protein (His tag) (ab217817)The purity of Human Serum Albumin, His Tag (ab217817) is more than 90% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
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Biological Activity - Recombinant Human Serum Albumin protein (His tag) (ab217817)Human Serum Albumin Protein, His Tag (ab217817) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag with an affinity constant of 0.381 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
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Functional Studies - Recombinant Human Serum Albumin protein (His tag) (ab217817)Bioactivity SPR: Immobilized biotinyated Huamn FcRN Heterodimer protein on SA chip can bind ab217817, his tag with an affitinity constant of 1.78μM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab217817 has been referenced in 1 publication.
- Srivastava A et al. The Linker Region Promotes Activity and Binding Efficiency of Modular LPMO towards Polymeric Substrate. Microbiol Spectr 10:e0269721 (2022). PubMed: 35080440
