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Recombinant Human SMAD5 protein (ab84651)

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SDS-PAGE - Recombinant Human SMAD5 protein (ab84651)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 85% Densitometry
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human SMAD5 protein

    • Purity

      > 85 % Densitometry.

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

    Specifications

    Our Abpromise guarantee covers the use of ab84651 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    General Info

    • Alternative names

      • DKFZp781C1895
      • DKFZp781O1323
      • Dwfc
      • hSmad5
      • JV5 1
      • JV5-1
      • MAD homolog 5
      • MAD, mothers against decapentaplegic homolog 5
      • MADH 5
      • MADH5
      • Mothers against decapentaplegic homolog 5
      • mothers against decapentaplegic, drosophila, homolog of, 5
      • Mothers against DPP homolog 5
      • MusMLP
      • SMA and MAD related protein 5
      • SMAD 5
      • SMAD family member 5
      • SMAD, mothers against DPP homolog 5
      • Smad5
      • SMAD5_HUMAN
      see all

    • Function

      Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the dwarfin/SMAD family.
      Contains 1 MH1 (MAD homology 1) domain.
      Contains 1 MH2 (MAD homology 2) domain.

    • Post-translational
      modifications

      Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
      Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human SMAD5 protein (ab84651)
      SDS-PAGE - Recombinant Human SMAD5 protein (ab84651)
      SDS-PAGE showing ab84651 at approximately 82kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab84651? Please let us know so that we can cite the reference in this datasheet.

    ab84651 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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